A map of nucleosome positions in yeast at base-pair resolution

被引：332

作者：

Brogaard, Kristin ^{[2
]}

Xi, Liqun ^{[1
]}

Wang, Ji-Ping ^{[1
]}

Widom, Jonathan ^{[2
,3
]}

机构：

[1] Northwestern Univ, Dept Stat, Evanston, IL 60208 USA

[2] Northwestern Univ, Dept Mol Biosci, Evanston, IL 60208 USA

[3] Northwestern Univ, Dept Chem, Evanston, IL 60208 USA

来源：

NATURE | 2012年 / 486卷 / 7404期

基金：

美国国家卫生研究院;

关键词：

CENTROMERIC NUCLEOSOME; DNA INTERACTIONS; CHROMATIN; TRANSCRIPTION; ORGANIZATION; SEQUENCE; HISTONE; FACILITATE; MECHANISM; CLEAVAGE;

D O I：

10.1038/nature11142

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The exact positions of nucleosomes along genomic DNA can influence many aspects of chromosome function. However, existing methods for mapping nucleosomes do not provide the necessary single-base-pair accuracy to determine these positions. Here we develop and apply a new approach for direct mapping of nucleosome centres on the basis of chemical modification of engineered histones. The resulting map locates nucleosome positions genome-wide in unprecedented detail and accuracy. It shows new aspects of the in vivo nucleosome organization that are linked to transcription factor binding, RNA polymerase pausing and the higher-order structure of the chromatin fibre.

引用

页码：496 / 501

页数：6

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