Determination of parishin, parishins B and C in traditional Chinese medicinal preparations by high performance liquid chromatography

High performance liquid chromatographic methods were established for the determination of parishin, parishins B and C in three kinds of the traditional Chinese medicinal prescriptions, including Gastrodiae Rhizoma: Shuen-Feng-Iun-Chi-San, Ban-Shia-Bai-Ju-Tian-Ma-Tang and Tsan-Siang-Tian-Ma-Tang. The samples were analyzed with an Inertsil ODS-2 reversed phase column by gradient elution with varied 0.1% (v/v) phosphoric acid-acetonitrile as mobile phases and detected at UV 222 nm. Ethoxybenzamide was used as an internal standard. Regression equations were derived showing linear relationships (correlation coefficients: 0.9976-0.9999) between the peak area ratios of each marker (parishin, parishins B and C) to ethoxybenzamide and concentration. The recoveries of parishin, parishins B and C from the Chinese medicinal preparations were 102.0-104.1%, 102.9-105.0% and 103.2-104.0%, respectively. The relative standard deviations of three marker constituents ranged between 0.90-2.47% (intraday) and 1.05-4.41% (interday). The contents of parishin, parishins B and C measured 1.58-2.85 mg/g, 1.58-3.25 mg/g and 1.45-1.72 mg/g, respectively.