Acid-induced unfolding of myoglobin triggered by a laser pH jump method

被引：34

作者：

Abbruzzetti, Stefania

Sottini, Silvia

Viappiani, Cristiano

Corrie, John E. T.

机构：

[1] Univ Parma, Dipartimento Fis, I-43100 Parma, Italy

[2] CNR, INFM, I-43100 Parma, Italy

[3] Natl Inst Med Res, MRC, London NW7 1AA, England

来源：

PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES | 2006年 / 5卷 / 06期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1039/b516533d

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using 1-(2-nitrophenyl)ethyl sulfate (caged sulfate) as a photoactivatable caged proton, we could induce complete acid unfolding of myoglobin with a single nanosecond laser pulse. This was possible because of the high (approximate to mM) concentration of protons released by the photolabile compound. The ability of the compound to produce a large pH jump arises because the other photoproducts (2-nitrosoacetophenone and sulfate ion) do not buffer the released protons. The complete time course of the unfolding kinetics, spanning a range from milliseconds to several seconds, could be accurately reproduced by monitoring absorbance changes in the visible spectrum at 633 nm.

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页码：621 / 628

页数：8

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