A conserved DYW domain of the pentatricopeptide repeat protein possesses a novel endoribonuclease activity

被引：72

作者：

Nakamura, Takahiro ^{[1
,2
]}

Sugita, Mamoru ^{[1
]}

机构：

[1] Nagoya Univ, Ctr Gene Res, Chikusa Ku, Nagoya, Aichi 4648602, Japan

[2] Kyushu Univ, Grad Sch Agr, Fukuoka 8128581, Japan

来源：

FEBS LETTERS | 2008年 / 582卷 / 30期

基金：

日本科学技术振兴机构; 日本学术振兴会;

关键词：

PPR protein; DYW domain; RNA editing; Endoribonuclease; RNase;

D O I：

10.1016/j.febslet.2008.11.017

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Many plant pentatricopeptide repeat (PPR) proteins are known to contain a highly conserved C-terminal DYW domain whose function is unknown. Recently, the DYW domain has been proposed to play a role in RNA editing in plant organelles. To address this possibility, we prepared recombinant DYW proteins and tested their cytidine deaminase activity. However, we could not detect any activity in the assays we used. Instead, we found that the recombinant DYW domains possessed endoribonuclease activity and cleaved before adenosine residues in the RNA molecule. Some DYW-containing PPR proteins may catalyze site-specific cleavage of target RNA species. (c) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：4163 / 4168

页数：6

共 26 条

[1] Genome-wide Insertional mutagenesis of Arabidopsis thaliana [J].

Alonso, JM ;

Stepanova, AN ;

Leisse, TJ ;

Kim, CJ ;

Chen, HM ;

Shinn, P ;

Stevenson, DK ;

Zimmerman, J ;

Barajas, P ;

Cheuk, R ;

Gadrinab, C ;

Heller, C ;

Jeske, A ;

Koesema, E ;

Meyers, CC ;

Parker, H ;

Prednis, L ;

Ansari, Y ;

Choy, N ;

Deen, H ;

Geralt, M ;

Hazari, N ;

Hom, E ;

Karnes, M ;

Mulholland, C ;

Ndubaku, R ;

Schmidt, I ;

Guzman, P ;

Aguilar-Henonin, L ;

Schmid, M ;

Weigel, D ;

Carter, DE ;

Marchand, T ;

Risseeuw, E ;

Brogden, D ;

Zeko, A ;

Crosby, WL ;

Berry, CC ;

Ecker, JR .

SCIENCE, 2003, 301 (5633) :653-657

[2] The multifarious roles of PPR proteins in plant mitochondrial gene expression [J].

Andres, Charles ;

Lurin, Claire ;

Small, Ian D. .

PHYSIOLOGIA PLANTARUM, 2007, 129 (01) :14-22

[3] CYTOSINE NUCLEOSIDE/NUCLEOTIDE DEAMINASES AND APOLIPOPROTEIN-B MESSENGER-RNA EDITING [J].

BHATTACHARYA, S ;

NAVARATNAM, N ;

MORRISON, JR ;

SCOTT, J ;

TAYLOR, WR .

TRENDS IN BIOCHEMICAL SCIENCES, 1994, 19 (03) :105-106

[4] The pentatricopeptide repeat gene OTP43 is required for trans-splicing of the mitochondrial nad1 intron 1 in Arabidopsis thaliana [J].

de Longevialle, Andeol Falcon ;

Meyer, Etienne H. ;

Andres, Charles ;

Taylor, Nicolas L. ;

Lurin, Claire ;

Millar, A. Harvey ;

Small, Ian D. .

PLANT CELL, 2007, 19 (10) :3256-3265

[5] Pentatricopeptide repeat (PPR) proteins as sequence-specificity factors in post-transcriptional processes in organelles [J].

Delannoy, E. ;

Stanley, W. A. ;

Bond, C. S. ;

Small, I. D. .

BIOCHEMICAL SOCIETY TRANSACTIONS, 2007, 35 :1643-1647

[6] Mapping of mitochondrial mRNA termini in Arabidopsis thaliana:: t-elements contribute to 5′ and 3′ end formation [J].

Forner, Joachim ;

Weber, Bärbel ;

Thuss, Sabine ;

Wildum, Steffen ;

Binder, Stefan .

NUCLEIC ACIDS RESEARCH, 2007, 35 (11) :3676-3692

[7] A nucleus-encoded factor, CRR2, is essential for the expression of chloroplast ndhB in Arabidopsis [J].

Hashimoto, M ;

Endo, T ;

Peltier, G ;

Tasaka, M ;

Shikanai, T .

PLANT JOURNAL, 2003, 36 (04) :541-549

[8] A pentatricopeptide repeat protein is required for RNA processing of clpP pre-mRNA in moss chloroplasts [J].

Hattori, Mitsuru ;

Miyake, Hiroshi ;

Sugita, Mamoru .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2007, 282 (14) :10773-10782

[9] Involvement of a site-specific trans-acting factor and a common RNA-binding protein in the editing of chloroplast mRNAs:: development of a chloroplast in vitro RNA editing system [J].

Hirose, T ;

Sugiura, M .

EMBO JOURNAL, 2001, 20 (05) :1144-1152

[10] Unique translation initiation at the second AUG codon determines mitochondrial localization of the phage-type RNA polymerases in the moss Physcomitrella patens [J].

Kabeya, Y ;

Sato, N .

PLANT PHYSIOLOGY, 2005, 138 (01) :369-382

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