MicroRNA-490-3p inhibits proliferation of A549 lung cancer cells by targeting CCND1

被引：64

作者：

Gu, Haihua ^{[1
]}

Yang, Tao ^{[1
]}

Fu, Shaozi ^{[1
]}

Chen, Xiaofan ^{[1
]}

Guo, Lei ^{[1
]}

Ni, Yiming ^{[1
]}

机构：

[1] Zhejiang Univ, Sch Med, Affiliated Hosp 1, Dept Cardiothorac Surg, Hangzhou 310003, Zhejiang, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2014年 / 444卷 / 01期

关键词：

miR-490-3p; CCND1; Proliferation; REGULATES CYCLIN D1; TUMOR-SUPPRESSOR; GROWTH; CDKS; MECHANISMS; EXPRESSION; ONCOGENE;

D O I：

10.1016/j.bbrc.2014.01.020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

MicroRNAs (miRNAs) are small non-coding RNAs that negatively regulate the translation of messenger RNAs by binding their 3'-untranslated region (3'UTR). In this study, we found that miR-490-3p is significantly down-regulated in A549 lung cancer cells compared with the normal bronchial epithelial cell line. To better characterize the role of miR-490-3p in A549 cells, we performed a gain-of-function analysis by transfecting the A549 cells with chemically synthesized miR-490-3P mimics. Overexpression of miR-490-3P evidently inhibits cell proliferation via G1-phase arrest. We also found that forced expression of miR-490-3P decreased both mRNA and protein levels of CCND1, which plays a key role in G1/S phase transition. In addition, the dual-luciferase reporter assays indicated that miR-490-3P directly targets CCND1 through binding its 3'UTR. These findings indicated miR-490-3P could be a potential suppressor of cellular proliferation. (C) 2014 Elsevier Inc. All rights reserved.

引用

页码：104 / 108

页数：5

共 32 条

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