X-ray imaging of various biological samples using a phase-contrast hard X-ray microscope

被引：25

作者：

Kim, Guk Bae ^{[2
]}

Yoon, Yae Jin ^{[3
]}

Shin, Tae Joo ^{[4
]}

Youn, Hwa Shik ^{[4
]}

Gho, Yong Song ^{[3
,5
]}

Lee, Sang Joon ^{[1
,2
]}

机构：

[1] Pohang Univ Sci & Technol, Dept Mech Engn, Pohang, South Korea

[2] Pohang Univ Sci & Technol, Syst Biodynam Natl Core Res Ctr, Pohang, South Korea

[3] Pohang Univ Sci & Technol, Sch Interdisciplinary Biosci & Bioengn, Pohang, South Korea

[4] Pohang Univ Sci & Technol, Pohang Accelerator Lab, Pohang, South Korea

[5] Pohang Univ Sci & Technol, Dept Life Sci, Pohang, South Korea

来源：

MICROSCOPY RESEARCH AND TECHNIQUE | 2008年 / 71卷 / 09期

关键词：

hard X-ray microscopy; phase-contrast; dehydrated cells; metastasized lung tissue;

D O I：

10.1002/jemt.20601

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

In this study, we visualized the internal structures of various bio-samples and found the optimum conditions of test samples for the 7 keV hard X-ray microscope of the Pohang light source. From the captured X-ray images, we could observe the intercellular and intracellular structures of dehydrated human cells and mouse tumor tissues without using any staining materials in a spatial resolution better than 100 nm. The metastasized lung tissue, which was several tens of micrometers in thickness, was found to be very well suited to this hard X-ray microscope system, because it is nearly impossible to observe such a nontransparent and thick sample with a high spatial resolution better than 100 nm using any microscopes such as a soft X-ray microscope, an optical microscope, or an electron microscope.

引用

页码：639 / 643

页数：5

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