cDNA cloning and expression of a novel serine protease in the mouse brain

被引：8

作者：

Suemoto, T ^{[1
]}

Taniguchi, M ^{[1
]}

Shiosaka, S ^{[1
]}

Yoshida, S ^{[1
]}

机构：

[1] Nara Inst Sci & Technol, Div Struct Cell Biol, Nara 6300101, Japan

来源：

MOLECULAR BRAIN RESEARCH | 1999年 / 70卷 / 02期

关键词：

granzyme K; hypothalamus; alternative initiation; in situ hybridization;

D O I：

10.1016/S0169-328X(99)00166-7

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A cDNA for a novel serine protease, termed brain type granzyme K (B-GRK) was cloned from the mouse brain. The cDNA codes a protein similar to granzyme K (GRK) but completely different at the N-terminus. Genomic Southern and PCR analysis of the gene suggests B-GRK is the alternative transcription form of GRK. B-GRK and GRK have a different organ-specific expression pattern: B-GRK is expressed in the brain, while GRK is expressed in the spleen. The recombinant fusion protein was detected in the neuro2a cells transfected with a plasmid containing B-GRK sequence. The mRNA for B-GRK/GRK was detected in cerebral cortex, hippocampus and diencephalon of the mouse brain. In situ hybridization for B-GRK/GRK revealed that several regions in the forebrain and hypothalamus express the mRNA. Developmental analysis showed that in the prenatal stage, the mRNA was expressed also in pituitary and pineal body in addition to the brain. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：273 / 281

页数：9

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