Studies of human and rat blood under oxidative stress: Changes in plasma thiol level, antioxidant enzyme activity, protein carbonyl content, and fluidity of erythrocyte membrane

被引：60

作者：

Yelinova, V ^{[1
]}

Glazachev, Y ^{[1
]}

Khramtsov, V ^{[1
]}

Kudryashova, L ^{[1
]}

Rykova, V ^{[1
]}

Salganik, R ^{[1
]}

机构：

[1] RUSSIAN ACAD SCI, INST CYTOL & GENET, NOVOSIBIRSK 630090, RUSSIA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1996年 / 221卷 / 02期

关键词：

D O I：

10.1006/bbrc.1996.0590

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

previously by selection and inbreeding of Wistar rats susceptible or resistant to the cataractogenic effect of galactose the S and R rat strains were developed as a model of oxidative stress. It was earlier found S rats have elevated level of OH-radical generation and enhanced lipid peroxidation compared with that for R rats. Our data show that superoxide dismutase and catalase activities in the blood of S mts are twice lower than corresponding values of R rats. Increased level of disulfides and protein carbonyl groups was obtained in the blood plasma of S-rats. It was found that fluidity of erythrocyte membrane measured:by photobleaching method is definitely higher for S rats compared with that for S rats. Similar effect of decreased catalase activity and increased disulfide content has been observed for kyphoscoliosis patients. (C) 1996 Academic Press, Inc.

引用

页码：300 / 303

页数：4

共 18 条

[1]

AEBI H, 1984, METHOD ENZYMOL, V105, P121

[2]

ALBERT V, 1995, METHOD ENZYMOL, V251, P448

[3]

HYDROLYSIS OF DISULFIDE BONDS IN WEAKLY ALKALINE MEDIA .1. OXIDIZED GLUTATHIONE [J].

ANDERSSO.LO ;

BERG, G .

BIOCHIMICA ET BIOPHYSICA ACTA, 1969, 192 (03) :534-&

[4]

INCREASE IN HEPATIC MIXED DISULFIDE AND GLUTATHIONE DISULFIDE LEVELS ELICITED BY PARAQUAT [J].

BRIGELIUS, R ;

LENZEN, R ;

SIES, H .

BIOCHEMICAL PHARMACOLOGY, 1982, 31 (08) :1637-1641

[5]

LIPID-PEROXIDATION CONTRIBUTES TO AGE-RELATED MEMBRANE RIGIDITY [J].

CHOE, M ;

JACKSON, C ;

YU, BP .

FREE RADICAL BIOLOGY AND MEDICINE, 1995, 18 (06) :977-984

[6]

DIKALOV SI, 1996, ARCH BIOCH BIOPHYS, V385

[7]

EREMENKO SI, 1986, BIOL MEMBRANY, V3, P253

[8]

FLOHE L, 1984, METHOD ENZYMOL, V105, P93

[9]

GLAZACHEFF YI, 1991, BIOL MEMBRANY, V8, P407

[10]

THE AGING PROCESS - MAJOR RISK FACTOR FOR DISEASE AND DEATH [J].

HARMAN, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (12) :5360-5363

← 1 2 →