Effects of Recombinant Human AMH on SCF Expression in Human Granulosa Cells

被引：16

作者：

Hu, Rong ^{[1
,2
]}

Lou, Yan ^{[2
]}

Wang, Fei-Miao ^{[2
]}

Ma, Hui-Ming ^{[2
]}

Wu, Xing ^{[2
]}

Zhang, Xiao-Mei ^{[3
]}

Li, Juan ^{[2
]}

Sun, Ying-Pu ^{[1
]}

机构：

[1] Zhengzhou Univ, Affiliated Hosp 1, Ctr Reprod Med, Zhengzhou 450052, Henan, Peoples R China

[2] Ningxia Med Univ, Gen Hosp, Natl Key Lab Fertil Preservat & Maintenance, Reprod Med Ctr,Minist Educ Ningxia, Yinchuan 750004, Peoples R China

[3] Yangzhou Univ, Northern Jiangsu Peoples Hosp, Dept Obstet & Gynecol, Reprod Med Ctr, Yangzhou 225001, Peoples R China

来源：

CELL BIOCHEMISTRY AND BIOPHYSICS | 2013年 / 67卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Anti-mullerian hormone; Granulosa cells; Stem Cell Factor; ANTI-MULLERIAN HORMONE; GROWTH;

D O I：

10.1007/s12013-013-9649-x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The aim of the present study was to investigate the effects of recombinant human anti-mullerian hormone (rhAMH) on Stem Cell Factor (SCF) expression in human granulosa cells (GCs). GCs were obtained from infertile patients undergoing IVF-ET cycles and cultured with 20 ng/ml of rhAMH. The levels of SCF mRNA and protein were detected in both matched and experimental group by real-time PCR, immunofluorescence, and ELISA, respectively, on day 4 of culture. We found that human GCs expressed SCF mRNA and protein, and SCF expression in the experimental group was significantly lower than that in the matched group (p < 0.05). We further showed that rhAMH inhibited SCF expression at mRNA and protein levels.

引用

页码：1481 / 1485

页数：5

共 14 条

[1]

Pollen-Specific SKP1-Like Proteins are Components of Functional SCF Complexes and Essential for Lily Pollen Tube Elongation [J].