Induction of apoptosis in rat myocardium under anoxic conditions

被引：9

作者：

Saprunova, VB ^{[1
]}

Kazimirchuk, SA ^{[1
]}

Tonshin, AA ^{[1
]}

Bakeeva, LE ^{[1
]}

Yagujinsky, LS ^{[1
]}

机构：

[1] Moscow MV Lomonosov State Univ, Belozersky Inst Physicochem Biol, Moscow 119899, Russia

来源：

BIOCHEMISTRY-MOSCOW | 2002年 / 67卷 / 02期

关键词：

apoptosis; anoxia; cardiomyocytes; myocardial infarction; mitochondria; electron microscopy;

D O I：

10.1023/A:1014426400741

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of anoxic incubation of small slices of isolated rat hearts on respiration, internucleosomal DNA fragmentation, and mitochondrial ultrastructure was investigated. Anoxic incubation for 72 h induced apoptosis accompanied by internucleosomal DNA fragmentation and changes in respiration and mitochondrial ultrastructure. The mitochondrial population was characterized by morphological heterogeneity. In a significant part of the mitochondrial population there were signs of mitochondrial swelling and appearance of electron-dense mitochondria. Anoxia also induced the appearance of an atypical (and previously unknown) population of small electron-dense mitochondria. They were characterized by unusual localization inside electron-light mitochondria. Under anoxic conditions the inner mitochondrial membrane formed electron-dense ordered structures. All changes described here reflect two opposing processes occurring in mitochondria: apoptotic destruction and compensatory processes responsible for maintenance of mitochondria.

引用

页码：246 / 253

页数：8

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