Comparative phylogeny of the ammonia monooxygenase subunit A and 16S rRNA genes of ammonia-oxidizing bacteria

被引：47

作者：

Aakra, Å ^{[1
]}

Utåker, JB ^{[1
]}

Nes, IF ^{[1
]}

机构：

[1] Agr Univ Norway, Dept Chem & Biotechnol, Lab Microbial Gene Technol, N-1432 As, Norway

来源：

FEMS MICROBIOLOGY LETTERS | 2001年 / 205卷 / 02期

关键词：

ammonia-oxidizing bacterium; comparative phylogeny; ammonia monooxygenase; 16S rRNA gene;

D O I：

10.1111/j.1574-6968.2001.tb10954.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A fragment of the ammonia monooxygenase gene (amoA) from 31 strains of ammonia-oxidizing bacteria (AOB) was sequenced and analysed phylogenetically. The results were compared with the phylogeny of 16S rDNA from AOB. For most groups of AOB we found a high consistency between the phylogenetic trees based on the 16S rDNA and amoA sequences. Although it is not a phylogenetic marker, using the amoA as a probe when studying microbial diversity will probably reduce the amount of non-AOB detected, compared to using rDNA based probes. The data presented in this paper extend and improve the basis for application of amoA in studies of AOB in the environment. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：237 / 242

页数：6

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