Exosomal microRNA in serum is a novel biomarker of recurrence in human colorectal cancer

被引：476

作者：

Matsumura, T. ^{[1
,2
]}

Sugimachi, K. ^{[1
]}

Iinuma, H. ^{[3
]}

Takahashi, Y. ^{[1
,2
]}

Kurashige, J. ^{[1
]}

Sawada, G. ^{[1
]}

Ueda, M. ^{[1
,2
]}

Uchi, R. ^{[1
]}

Ueo, H. ^{[1
]}

Takano, Y. ^{[1
]}

Shinden, Y. ^{[1
]}

Eguchi, H. ^{[1
]}

Yamamoto, H. ^{[2
]}

Doki, Y. ^{[2
]}

Mori, M. ^{[2
]}

Ochiya, T. ^{[4
]}

Mimori, K. ^{[1
]}

机构：

[1] Kyushu Univ, Dept Surg, Beppu Hosp, Beppu, Oita 8740838, Japan

[2] Osaka Univ, Grad Sch Med, Dept Surg Gastroenterol, Suita, Osaka 5650871, Japan

[3] Teikyo Univ, Dept Surg, Sch Med, Itabashi Ku, Tokyo 1730003, Japan

[4] Natl Canc Ctr, Res Inst, Div Mol & Cellular Med, Tokyo 1040045, Japan

来源：

BRITISH JOURNAL OF CANCER | 2015年 / 113卷 / 02期

基金：

日本科学技术振兴机构; 日本学术振兴会;

关键词：

exosome; microRNA; miR-17-92a cluster; miR-19a; biomarker; colorectal cancer; EXTRACELLULAR VESICLES; GENE-EXPRESSION; IDENTIFICATION; METASTASIS; RESISTANCE; DIAGNOSIS; MIR-17-92; THERAPY; CLUSTER; CELLS;

D O I：

10.1038/bjc.2015.201

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

Background: Functional microRNAs (miRNAs) in exosomes have been recognised as potential stable biomarkers in cancers. The aim of this study is to identify specific miRNAs in exosome as serum biomarkers for the early detection of recurrence in human colorectal cancer (CRC). Methods: Serum samples were sequentially obtained from six patients with and without recurrent CRC. The miRNAs were purified from exosomes, and miRNA microarray analysis was performed. The miRNA expression profiles and copy number aberrations were explored using microarray and array CGH analyses in 124 CRC tissues. Then, we validated exosomal miRNAs in 2 serum sample sets (90 and 209 CRC patients) by quantitative real-time RT-PCR. Results: Exosomal miR-17-92a cluster expression level in serum was correlated with the recurrence of CRC. Exosomal miR-19a expression levels in serum were significantly increased in patients with CRC as compared with healthy individuals with gene amplification. The CRC patients with high exosomal miR-19a expression showed poorer prognoses than the low expression group (P<0.001). Conclusions: Abundant expression of exosomal miR-19a in serum was identified as a prognostic biomarker for recurrence in CRC patients.

引用

页码：275 / 281

页数：7

共 35 条

[1]

Recent approaches to identifying biomarkers for high-risk stage II colon cancer [J].