PslD is a secreted protein required for biofilm formation by Pseudomonas aeruginosa

被引：43

作者：

Campisano, A ^{[1
]}

Schroeder, C ^{[1
]}

Schemionek, M ^{[1
]}

Overhage, J ^{[1
]}

Rehm, BHA ^{[1
]}

机构：

[1] Massey Univ, Inst Mol Sci, Palmerston North, New Zealand

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2006年 / 72卷 / 04期

关键词：

D O I：

10.1128/AEM.72.4.3066-3068.2006

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The function of pslD, which is part of the psl operon from Pseudomonas aeruginosa, was investigated in this study. The psl operon is involved in exopolysaccharide biosynthesis and biofilm formation. An isogenic marker-free pslD deletion mutant of P. aeruginosa PAO1. which was deficient in the formation of differentiated biofilms was generated. Expression of only the pslD gene coding region restored the wild-type phenotype. A C-terminal, hexahistidine tag fusion enabled the identification of PslD. LacZ and PhoA translational fusions with PslD indicated that PslD is a secreted protein required for biofilm formation, presumably via its role in exopolysaccharide export.

引用

页码：3066 / 3068

页数：3

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