Synchrony in Human, Mouse and Bacterial Cell Cultures A Comparison

被引：31

作者：

Helmstetter, Charles E. ^{[1
]}

Thornton, Maureen ^{[1
]}

Romero, Ana ^{[1
]}

Eward, K. Leigh ^{[1
]}

机构：

[1] Florida Inst Technol, Dept Biol Sci, Melbourne, FL 32901 USA

来源：

CELL CYCLE | 2003年 / 2卷 / 01期

关键词：

Synchronous growth; Cell cycle;

D O I：

10.4161/cc.2.1.185

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Growth characteristics of synchronous human MOLT-4, human U-937 and mouse L1210 cultures produced with a new minimally-disturbing technology were compared to each other and to synchronous Escherichia coli B/r. Based on measurements of cell concentrations during synchronous growth, synchrony persisted in similar fashion for all cells. Cell size and DNA distributions in the mammalian cultures also progressed synchronously and reproducibly for multiple cell cycles. The results demonstrate that unambiguous multi-cycle synchrony, critical for verifying the absence of significant growth imbalances induced by the synchronization procedure, is feasible with these cell lines, and possibly others.

引用

页码：42 / 45

页数：4

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