Novel near-infrared cyanine fluorochromes: Synthesis, properties, and bioconjugation

被引：139

作者：

Lin, YH ^{[1
]}

Weissleder, R ^{[1
]}

Tung, CH ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Massachusetts Gen Hosp, Ctr Mol Imaging Res, Charlestown, MA 02129 USA

来源：

BIOCONJUGATE CHEMISTRY | 2002年 / 13卷 / 03期

关键词：

D O I：

10.1021/bc0155723

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Recently, near-infrared (NIR) fluorescence light has been applied to image various biological events in vivo, because it penetrates tissue more efficiently than light in the visible spectrum. Compounds exhibiting fluorescent properties in the NIR range are key elements for this upcoming optical imaging technology. In this paper, we report the synthesis of four new, water-soluble NIR cyanine fluorochromes which have superior chemical stability and optical properties. Each fluorochrome was designed with a monoreactive carboxyl group for labeling purposes. When multiple fluorochromes were attached to a single macromolecule, fluorescence quenching was observed. On the basis of this property, a novel autoquenched enzyme sensitive NIR fluorescence probe was prepared.

引用

页码：605 / 610

页数：6

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