Rapid cytocidal and cytostatic chemosensitivity test by measuring total amount of mRNA

被引：5

作者：

Miura, Y

deFries, R

Shimada, H

Mitsuhashi, M

机构：

[1] UNIV CALIF IRVINE,DEPT PATHOL,IRVINE,CA 92717

[2] YOKOHAMA CITY UNIV,SCH MED,DEPT SURG 2,YOKOHAMA,KANAGAWA 232,JAPAN

[3] HITACHI CHEM RES CTR,DIV MED SCI,IRVINE,CA 92715

来源：

CANCER LETTERS | 1997年 / 116卷 / 02期

关键词：

chemosensitivity; cancers; mRNA; Yoyo-1; MTT;

D O I：

10.1016/S0304-3835(97)00191-2

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Chemosensitivity of vinblastin, cisplatin, and mitomycin C were assessed in four different human cancer cell lines (U937, HL-60, CaR-1, and HepG2) by MTT (3-(4,5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide) assay and the measurement of total cytosolic poly(A) + mRNA. Results of 12 h mRNA assay with 10 x peak plasma concentration (PPC) were significantly correlated with that of standard 3 days MTT assay with 1 x PPC. Furthermore, mRNA assay was changed more significantly than MTT assay under cytostatic condition. Because of its minimum culture requirement (12 h) and broad spectrum (cytocidal and cytostatic), mRNA assay will become a useful tool for chemosensitivity test. (C) 1997 Elsevier Science Ireland Ltd.

引用

页码：139 / 144

页数：6

共 13 条

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