Evaluation of dipicolinic acid for detection of IMP- or VIM-type metallo-β-lactamase-producing Pseudomonas aeruginosa clinical isolates

被引：39

作者：

Kimura, S ^{[1
]}

Ishii, Y ^{[1
]}

Yamaguchi, K ^{[1
]}

机构：

[1] Toho Univ, Sch Med, Dept Microbiol & Infect Dis, Tokyo 1438540, Japan

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2005年 / 53卷 / 03期

关键词：

metallo-beta-lactamase; dipicolinic acid; IMP; VIM;

D O I：

10.1016/j.diagmicrobio.2005.05.017

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

We evaluated dipicolinic acid (DPA) as a chelating agent for detection of IMP- or VIM-type metallo-beta-lactamase (MBL)-producing Pseudomonas aeruginosa clinical isolates. Using the broth microdilution testing in the presence or absence of DPA, MBL producers exhibited 100%, 92%, or 100% of >= 8 times (media, 32 times) reduction of MICs in presence of DPA for ceftazidime, imipenem, or meropenem, respectively. In disk diffusion testing, expansion of growth inhibitory zone of these clinical isolates was clearly observed. Thus, DPA could be useful in the detection for MBL-producing P. aeruginosa clinical isolates. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：241 / 244

页数：4

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