Cell proliferation in ependymal/subventricular zone and nNOS expression following focal cerebral ischemia in adult rats

被引：45

作者：

Zhang, PB ^{[1
]}

Liu, Y ^{[1
]}

Li, J ^{[1
]}

Kang, QY ^{[1
]}

Tian, YF ^{[1
]}

Chen, XL ^{[1
]}

Shi, QD ^{[1
]}

Song, TS ^{[1
]}

机构：

[1] Xian Jiaotong Univ, Sch Med, Ctr Res Neurosci, Environm & Genes Related Dis Key Lab,Educ Minist, Xian 710061, Peoples R China

来源：

NEUROLOGICAL RESEARCH | 2006年 / 28卷 / 01期

关键词：

neurogenesis; neuronal nitric oxide synthase; focal cerebral ischemia; rat;

D O I：

10.1179/016164106X91942

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

Neuronal nitric oxide synthase (nNOS) regulates neurogenesis in normal developing brain, but the role of nNOS in neurogenesis in the ischemic brain remains unclear. To investigate the temporal and spatial relationship between cell proliferation of the ependymal/subventricular zone (SVZ), a principal neuroproliferative region in the adult brain, and nNOS expression, the male Sprague-Dawley rats weighing 250-350 g were used. The focal cerebral ischemia was induced by middle cerebral artery occlusion (MCAO). 10 mu l of 0.2% fluorescence dye Dil was injected into the right lateral ventricle to prelabel ependymal/subventricular zone cells before ischemia. The rats were killed immediately after ischemia and days 1, 3, 7, 11, 14, 21 and 28 after ischemia. Dil-labeled cell counting was employed to assess cell proliferation. Immunohistochemistry and grayscale analysis were performed to determine nNOS localization and its quantity in the specific regions. Compared with control, the density of Dil-labeled cells in the ipsilateral ependyma/SVZ was significantly higher at days 1, 3, 7 and 11 after ischemia, whereas the quantity of nNOS expression in the ependyma/SVZ adjacent regions was significantly lower at the above time points. Additionally, nNOS positive cells were largely excluded from SVZ, and their long processes did not enter the ependyma/SVZ. Our results indicate that after focal cerebral ischemia, decreased nNOS expression in the ipsilateral ependymal/SVZ adjacent regions might be related to cell proliferation in the ependymal/SVZ.

引用

页码：91 / 96

页数：6

共 25 条

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