DNA fingerprinting of Cannabis sativa using inter-simple sequence repeat (ISSR) amplification

被引：47

作者：

Kojoma, M

Iida, O

Makino, Y

Sekita, S

Satake, M

机构：

[1] RIKEN, Inst Phys & Chem Res, Plant Funct Lab, Wako, Saitama 3510198, Japan

[2] Natl Inst Hlth Sci, Izu Expt Stn Med Plants, Shizuoka, Japan

[3] Natl Inst Hlth Sci, Div Pharmacognosy & Phytochem, Tokyo 158, Japan

来源：

PLANTA MEDICA | 2002年 / 68卷 / 01期

关键词：

Cannabis sativa; hemp; Cannabaceae; DNA profiling; ISSR; cannabinoid; HPLC;

D O I：

10.1055/s-2002-19875

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Chemical analysis of cannabinoid, and Inter-Simple Sequence Repeat (ISSR) fingerprinting of DNA were used to identify different samples of Cannabis sativa L for forensic purposes. Three samples were classified into two types, tetrahydrocannabinol (THC) and cannabidiol (CBD) chemo-types, by high performance liquid chromatography (HPLC). The two samples of the CBD type were not distinguished by their HPLC patterns. ISSR Fingerprinting identified polymorphic DNA patterns between these samples. ISSR fingerprinting clearly differentiated between cannabis samples that could riot be achieved by HPLC analysis.

引用

页码：60 / 63

页数：4

共 20 条

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