Voltage-dependent interaction of the peptaibol antibiotic zervamicin II with phospholipid vesicles

被引：12

作者：

Kropacheva, TN

Raap, J

机构：

[1] Leiden Univ, Gorlaeus Labs, Inst Chem, NL-2300 RA Leiden, Netherlands

[2] Udmurt State Univ, Dept Chem, Izhevsk, Russia

来源：

FEBS LETTERS | 1999年 / 460卷 / 03期

关键词：

ion channel; transmembrane potential; phosphatidylcholine vesicle;

D O I：

10.1016/S0014-5793(99)01401-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of a transmembrane potential on ion channel formation by zervamicin II (ZER-II) was studied in a vesicular model system, The dissipation of diffusion potential caused by addition of ZER-II to small phosphatidylcholine vesicles mas monitored using fluorescent (Safranine T) and optical (Oxonol YI) probes. Cis-positive potentials facilitated channel formation, while at cis-negative potentials, ion fluxes were inhibited. A potential-independent behavior of ZER-II was observed at high peptide concentrations, most likely due to its membrane modifying property. (C) 1999 Federation of European Biochemical Societies.

引用

页码：500 / 504

页数：5

共 18 条

[1] TRICHOGIN-A-IV, AN 11-RESIDUE LIPOPEPTAIBOL FROM TRICHODERMA-LONGIBRACHIATUM [J].