Design and evaluation of various methods for the construction of kinetic performance limit plots for supercritical fluid chromatography

被引:24
作者
Delahaye, Sander [1 ]
Broeckhoven, Ken [2 ]
Desmet, Gert [2 ]
Lynen, Frederic [1 ]
机构
[1] Univ Ghent, Dept Organ Chem, Separat Sci Grp, B-9000 Ghent, Belgium
[2] Vrije Univ Brussel, Dept Chem Engn, B-1050 Brussels, Belgium
关键词
Supercritical fluid chromatography; Kinetic performance; Variable column length method; Isopycnic method; Comparison with HPLC; PHASE LIQUID-CHROMATOGRAPHY; CARBON-DIOXIDE; ISOPYCNIC PLOTS; COLUMN EFFICIENCY; PRESSURE-DROP; MOBILE-PHASE; SOLUTE RETENTION; CRITICAL REGION; PACKED-COLUMNS; PARTICLE-SIZE;
D O I
10.1016/j.chroma.2012.08.040
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Supercritical fluid chromatography (SFC) is attributed many advantages over high performance liquid chromatography (HPLC). Next to the fact that SFC is greener than HPLC, which is especially important for preparative separations, SFC is claimed to be able to deliver faster separations at higher efficiencies (N) than HPLC. This is due to the higher diffusitivity of analytes in supercritical fluids compared to liquids (higher optimum mobile phase velocity) and to the lower viscosity of the mobile phases in SFC compared to HPLC, which results in smaller pressure drops allowing the use of longer columns and/or columns packed with smaller particles at higher velocities. In order to quantify this claimed kinetic performance advantage, it is essential to construct unbiased kinetic plots to make the comparison between HPLC and SFC. The high compressibility of the mobile phase in SFC however makes this problematic. A variable column length (L) kinetic plot method is therefore developed in this work. Because the pressure history in the column is kept constant for every data point in this method, this way of working definitely delivers exact values for the kinetic performance limits in SFC. It is shown that the traditional way of measuring the performance as a function of flow rate (fixed back pressure and column length) cannot deliver the same correct results as this variable L method. However, the isopycnic way of working on a fixed column length has also been proven to be a good alternative for the expensive and time consuming variable L method. Finally, isopycnic kinetic plots are used to compare SFC and HPLC performance in a quantitative way. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:152 / 160
页数:9
相关论文
共 47 条
[1]   The kinetic plot method applied to gradient chromatography: Theoretical framework and experimental validation [J].
Broeckhoven, K. ;
Cabooter, D. ;
Lynen, F. ;
Sandra, P. ;
Desmet, G. .
JOURNAL OF CHROMATOGRAPHY A, 2010, 1217 (17) :2787-2795
[2]   Corona-charged aerosol detection in supercritical fluid chromatography for pharmaceutical analysis [J].
Brunelli, C. ;
Gorecki, T. ;
Zhao, Y. ;
Sandra, P. .
ANALYTICAL CHEMISTRY, 2007, 79 (06) :2472-2482
[3]   Development of a supercritical fluid chromatography high-resolution separation method suitable for pharmaceuticals using cyanopropyl silica [J].
Brunelli, Claudio ;
Zhao, Yining ;
Brown, Melissa-Hanna ;
Sandra, Pat .
JOURNAL OF CHROMATOGRAPHY A, 2008, 1185 (02) :263-272
[4]   Effect of Pressure, Particle Size, and Time on Optimizing Performance in Liquid Chromatography [J].
Carr, Peter W. ;
Wang, Xiaoli ;
Stoll, Dwight R. .
ANALYTICAL CHEMISTRY, 2009, 81 (13) :5342-5353
[5]  
Cole J, 2008, LC GC EUR, P44
[6]   Effect of analyte properties on the kinetic performance of liquid chromatographic separations [J].
de Villiers, Andre ;
Lynen, Frederic ;
Sandra, Pat .
JOURNAL OF CHROMATOGRAPHY A, 2009, 1216 (16) :3431-3442
[7]   Geometry-independent plate height representation methods for the direct comparison of the kinetic performance of LC supports with a different size or morphology [J].
Desmet, G ;
Clicq, D ;
Gzil, P .
ANALYTICAL CHEMISTRY, 2005, 77 (13) :4058-4070
[8]   Improved separation of furocoumarins of essential oils by supercritical fluid chromatography [J].
Desmortreux, C. ;
Rothaupt, M. ;
West, C. ;
Lesellier, E. .
JOURNAL OF CHROMATOGRAPHY A, 2009, 1216 (42) :7088-7095
[9]   Kinetic performance of reversed-phase C18 high-performance liquid chromatography columns compared by means of the Kinetic Plot Method in pharmaceutically relevant applications [J].
Fanigliulo, A. ;
Cabooter, D. ;
Bellazzi, G. ;
Allieri, B. ;
Rottigni, A. ;
Desmet, G. .
JOURNAL OF CHROMATOGRAPHY A, 2011, 1218 (21) :3351-3359
[10]   VISCOSITY ESTIMATION IN BINARY AND TERNARY SUPERCRITICAL FLUID MIXTURES CONTAINING CARBON DIOXIDE USING A SUPERCRITICAL FLUID CHROMATOGRAPH [J].
Fields, P. R. ;
Chester, T. L. ;
Stalcup, A. M. .
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2011, 34 (12) :995-1003