Quantification of surfactin in culture supernatants by hemolytic activity

被引：50

作者：

Morán, AC

Martínez, MA

Siñeriz, F

机构：

[1] PROIMI Biotecnol Planta Piloto Proc Ind Microbiol, San Miguel De Tucuman, Argentina

[2] Univ Nacl Tucuman, Fac Bioquim Quim & Farm, Catedra Microbiol Super, RA-4000 San Miguel De Tucuman, Tucuman, Argentina

来源：

BIOTECHNOLOGY LETTERS | 2002年 / 24卷 / 03期

关键词：

Bacillus subtilis; biosurfactant; hemolysis; surfactin;

D O I：

10.1023/A:1014140820207

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

A quantitative assay for measuring surfactin in culture supernatants was developed based on its ability to cause hemolysis. The presence of 145 ml ethanol l(-1) at 37 degreesC in reaction mixture gave an optimal sensitivity. Compared with measurements of dry weights of surfactin, the hemolytic assay gave more reliable concentration values because it did not involve separation steps from the supernatant. The hemolytic assay herein proposed showed to be a simple and low-cost method to readily assess biosurfactants in liquid cultures.

引用

页码：177 / 180

页数：4

共 10 条

[1]

THE ISOLATION OF A THERMOPHILIC BIOSURFACTANT PRODUCING BACILLUS SP [J].