Sortases and the art of anchoring proteins to the envelopes of gram-positive bacteria

被引:524
作者
Marraffini, LA
DeDent, AC
Schneewind, O
机构
[1] Univ Chicago, Dept Microbiol Genet & Cell Biol, Chicago, IL 60637 USA
[2] Univ Chicago, Dept Microbiol, Chicago, IL 60637 USA
关键词
D O I
10.1128/MMBR.70.1.192-221.2006
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The cell wall envelopes of gram-positive bacteria represent a surface organelle that not only functions as a cytoskeletal element but also promotes interactions between bacteria and their environment. Cell wall peptidoglycan is covalently and noncovalently decorated with teichoic acids, polysaccharides, and proteins. The sum of these molecular decorations provides bacterial envelopes with species- and strain-specific properties that are ultimately responsible for bacterial virulence, interactions with host immune systems, and the development of disease symptoms or successful outcomes of infections. Surface proteins typically carry two topogenic sequences, i.e., N-terminal signal peptides and C-terminal sorting signals. Sortases catalyze a transpeptidation reaction by first cleaving a surface protein substrate at the cell wall. sorting signal. The resulting acyl enzyme intermediates between sortases and their substrates are then resolved by the nucleophilic attack of amino groups, typically provided by the cell wall cross bridges of peptidoglycan precursors. The surface protein linked to peptidoglycan is then incorporated into the envelope and displayed on the microbial surface. This review focuses on the mechanisms of surface protein anchoring to the cell wall envelope by sortases and the role that these enzymes play in bacterial physiology and pathogenesis.
引用
收藏
页码:192 / +
页数:31
相关论文
共 229 条
[1]   ANALYSIS OF SIGNALS FOR SECRETION IN THE STAPHYLOCOCCAL PROTEIN-A GENE [J].
ABRAHMSEN, L ;
MOKS, T ;
NILSSON, B ;
HELLMAN, U ;
UHLEN, M .
EMBO JOURNAL, 1985, 4 (13B) :3901-3906
[2]   IDENTIFICATION OF ACETYLCHOLINE-RECEPTOR CHANNEL-LINING RESIDUES IN THE M1 SEGMENT OF THE ALPHA-SUBUNIT [J].
AKABAS, MH ;
KARLIN, A .
BIOCHEMISTRY, 1995, 34 (39) :12496-12500
[3]   VIRULENCE OF STAPHYLOCOCCUS-AUREUS MUTANTS ALTERED IN TYPE-5 CAPSULE PRODUCTION [J].
ALBUS, A ;
ARBEIT, RD ;
LEE, JC .
INFECTION AND IMMUNITY, 1991, 59 (03) :1008-1014
[4]   The YSIRK-G/S motif of staphylococcal protein A and its role in efficiency of signal peptide processing [J].
Bae, T ;
Schneewind, O .
JOURNAL OF BACTERIOLOGY, 2003, 185 (09) :2910-2919
[5]   Progress toward characterization of the group a Streptococcus metagenome:: Complete genome sequence of a macrolide-resistant serotype M6 strain [J].
Banks, DJ ;
Porcella, SF ;
Barbian, KD ;
Beres, SB ;
Philips, LE ;
Voyich, JM ;
DeLeo, FR ;
Martin, JM ;
Somerville, GA ;
Musser, JM .
JOURNAL OF INFECTIOUS DISEASES, 2004, 190 (04) :727-738
[6]   A novel sortase, SrtC2, from Streptococcus pyogenes anchors a surface protein containing a QVPTGV motif to the cell wall [J].
Barnett, TC ;
Patel, AR ;
Scott, JR .
JOURNAL OF BACTERIOLOGY, 2004, 186 (17) :5865-5875
[7]   Differential recognition of surface proteins in Streptococcus pyogenes by two sortase gene homologs [J].
Barnett, TC ;
Scott, JR .
JOURNAL OF BACTERIOLOGY, 2002, 184 (08) :2181-2191
[8]   Intracellular Staphylococcus aureus escapes the endosome and induces apoptosis in epithelial cells [J].
Bayles, KW ;
Wesson, CA ;
Liou, LE ;
Fox, LK ;
Bohach, GA ;
Trumble, WR .
INFECTION AND IMMUNITY, 1998, 66 (01) :336-342
[9]   Genomic localization of a T serotype locus to a recombinatorial zone encoding extracellular matrix-binding proteins in Streptococcus pyogenes [J].
Bessen, DE ;
Kalia, A .
INFECTION AND IMMUNITY, 2002, 70 (03) :1159-1167
[10]   Inactivation of the srtA gene in Listeria monocytogenes inhibits anchoring of surface proteins and affects virulence [J].
Bierne, H ;
Mazmanian, SK ;
Trost, M ;
Pucciarelli, MG ;
Liu, G ;
Dehoux, P ;
Jänsch, L ;
Garcia-del Portillo, F ;
Schneewind, O ;
Cossart, P .
MOLECULAR MICROBIOLOGY, 2002, 43 (04) :869-881