Physical Inactivity Differentially Alters Dietary Oleate and Palmitate Trafficking

被引：82

作者：

Bergouignan, Audrey ^{[1
]}

Trudel, Guy ^{[2
]}

Simon, Chantal ^{[3
]}

Chopard, Angele ^{[4
,5
]}

Schoeller, Dale A. ^{[6
]}

Momken, Iman ^{[1
]}

Votruba, Susanne B. ^{[7
]}

Desage, Michel ^{[8
]}

Burdge, Graham C. ^{[9
]}

Gauquelin-Koch, Guillemette ^{[10
]}

Normand, Sylvie ^{[8
]}

Blanc, Stephane ^{[1
]}

机构：

[1] Louis Pasteur Univ, Dept Ecol Physiol & Ethol, Hubert Curten Pluridisciplinary Inst, CNRS,UMR 7178, Strasbourg, France

[2] Univ Ottawa, Dept Med, Bone & Joint Res Lab, Ottawa, ON, Canada

[3] Louis Pasteur Univ, Dept Nutr, Hautepierre Hosp, Strasbourg, France

[4] Nice Sophia Antipolis Univ, Physiol Adapt Lab, Nice, France

[5] Univ Ottawa, Dept Cellular & Mol Med, Ottawa, ON, Canada

[6] Univ Wisconsin, Dept Nutr Sci, Madison, WI 53706 USA

[7] NIDDKD, Obes & Diabet Res Sect, NIH, Phoenix, AZ USA

[8] Human Nutr Res Ctr Rhone Alpes CNRHL, Laennec Med Fac, Lyon, France

[9] Univ Southampton, Inst Human Nutr, Southampton, Hants, England

[10] French Space Agcy CNES, Paris, France

来源：

DIABETES | 2009年 / 58卷 / 02期

基金：

加拿大健康研究院;

关键词：

FREE FATTY-ACIDS; ACTIVATED PROTEIN-KINASE; SKELETAL-MUSCLE; BED REST; TRIACYLGLYCEROL SYNTHESIS; ENERGY-EXPENDITURE; OXIDATION; EXERCISE; LIVER; METABOLISM;

D O I：

10.2337/db08-0263

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

OBJECTIVE-Obesity and diabetes are characterized by the incapacity to use fat as fuel. We hypothesized that this reduced fat oxidation is secondary to a sedentary lifestyle. RESEARCH DESIGN AND METHODS-We investigated the effect of a 2-month bed rest on the dietary oleate and palmitate trafficking in lean women (control group, n = 8) and the effect of concomitant resistance/aerobic exercise training as a counter-measure (exercise group, n = 8). Trafficking of stable isotope-labeled dietary fats was combined with muscle gene expression and magnetic resonance imaging-derived muscle fat content. analyses. RESULTS-In the control group, bed rest increased the cumulative [1-C-13]oleate and [d(31)]palmitate appearance in triglycerides (37%, P = 0.009, and 34%, P = 0.016, respectively) and nonesterified fatty acids (NEFAs) (37%, P = 0.038, and 38%, P = 0.002) and decreased muscle lipoprotein lipase (P = 0.043) and fatty acid translocase CD36 (P = 0.043) mRNA expressions. Plasma NEFA-to-triglyceride ratios for [1-C-13]oleate and [d(31)]palmitate remained unchanged, suggesting that the same proportion of tracers enters the peripheral tissues after bed rest. Bed rest did not affect [1-C-13]oleate oxidation but decreased [d(31)]palmitate oxidation by -8.2 +/- 4.9% (P < 0.0001). Despite a decreased spontaneous energy intake and a reduction of 1.9 +/- 0.3 kg (P = 0.001) in fat mass, exercise training did not mitigate these alterations but partially maintained fat-free mass, insulin sensitivity, and total lipid oxidation in fasting and fed states. In both groups, muscle fat content increased by 2.7% after bed rest and negatively correlated with the reduction in [d(31)]palmitate oxidation (r(2) = 0.48, P = 0.003). CONCLUSIONS-While saturated and monounsaturated fats have similar plasma trafficking and clearance, physical inactivity affects the partitioning of saturated fats toward storage, likely leading to an accumulation of palmitate in muscle fat. Diabetes 58:367-376, 2009

引用

页码：367 / 376

页数：10

共 55 条

[1] Optimizing fat oxidation through exercise and diet [J].