Determination of ethylenediaminetetraacetic acid in foods by reversed-phase high-performance liquid chromatography

被引：20

作者：

Kemmei, Tomoko ^{[1
]}

Kodama, Shuji ^{[1
]}

Yamamoto, Atsushi ^{[2
]}

Inoue, Yoshinori ^{[3
]}

Hayakawa, Kazuichi ^{[4
]}

机构：

[1] Toyama Inst Hlth, Imizu, Toyama 9390363, Japan

[2] Chubu Univ, Dept Biol Chem, Coll Biosci & Biotechnol, Kasugai, Aichi 4878501, Japan

[3] Nippon Filcon Co LTD, Prod Planning & Dev Dep R&D Ctr, Inagi 2068577, Japan

[4] Kanazawa Univ, Inst Med Pharmaceut & Hlth Sci, Kanazawa, Ishikawa 9201192, Japan

来源：

FOOD CHEMISTRY | 2013年 / 138卷 / 2-3期

基金：

日本学术振兴会;

关键词：

EDTA; Reversed-phase HPLC; Food additives; UV detection; PHARMACEUTICAL FORMULATIONS; EXTRACTION; EDTA;

D O I：

10.1016/j.foodchem.2012.11.103

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

A convenient HPLC method for the quantitative determination of EDTA in foods was developed. EDTA in food samples was easily extracted with water by ultrasonication. After converting to Fe(III) complex in the presence of Fe(III) ions, EDTA was separated on a reversed-phase 00 column and detected with ultraviolet detection (260 nm). Citrate and malate, which are present in many foods, also formed Fe(III) complexes but they did not interfere the chromatographic detection of EDTA. The method allowed determination of EDTA in foods at concentrations as low as 0.01 mmol/kg. Good recoveries (95.2-101%) were obtained by the standard addition method on four samples with high repeatability (RSD, 0.8-3.4%). The method was successfully applied to the analysis of EDTA in carbonated drinks, jellies, canned beans, canned corn and food supplements. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：866 / 869

页数：4

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