Oxygen-glucose deprivation of neurons transfected with toll-like receptor 3-siRNA

被引：4

作者：

Cui, Guiyun ^{[1
]}

Wang, Xiaopeng ^{[1
]}

Ye, Xinchun ^{[1
]}

Zu, Jie ^{[1
]}

Zan, Kun ^{[1
]}

Hua, Fang ^{[1
]}

机构：

[1] Xuzhou Med Coll, Affiliated Hosp, Dept Neurol, Xuzhou 221002, Jiangsu, Peoples R China

来源：

NEURAL REGENERATION RESEARCH | 2013年 / 8卷 / 34期

基金：

中国国家自然科学基金;

关键词：

neural regeneration; brain injury; Toll-like receptor 3; small interfering RNA; primary neurons; ischemia/reperfusion injury; transfection; hypoxia; liposome; immune/inflammatory reactions; RNA interference; grants-supported paper; neuroregeneration; DOUBLE-STRANDED-RNA; INFLUENCES SIRNA EFFICACY; OXIDATIVE STRESS; INNATE IMMUNITY; MESSENGER-RNA; GENE-TRANSFER; BRAIN-INJURY; ACTIVATION; ISCHEMIA; INTERFERENCE;

D O I：

10.3969/j.issn.1673-5374.2013.34.007

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Toll-like receptor 3 protein expression has been shown to be upregulated during cerebral ischemia/reperfusion injury in rats. In this study, rat primary cortical neurons were subjected to oxygen-glucose deprivation to simulate cerebral ischemia/reperfusion injury. Chemically synthesized small interfering RNA (siRNA)-1280, -1724 and -418 specific to toll-like receptor 3 were transfected into oxygen-glucose deprived cortical neurons to suppress the upregulation of toll-like receptor 3 protein expression. Western blotting demonstrated that after transfection with siRNA, toll-like receptor 3 protein expression reduced, especially in the toll-like receptor 3-1724 group. These results suggested that siRNA-1724 is an optimal sequence for inhibiting toll-like receptor 3 expression in cortical neurons following oxygen-glucose deprivation.

引用

页码：3233 / 3240

页数：8

共 59 条

[1]

Toll-like receptors in the induction of the innate immune response [J].

Aderem, A ;

Ulevitch, RJ .

NATURE, 2000, 406 (6797) :782-787

[2]

Recognition of double-stranded RNA and activation of NF-κB by Toll-like receptor 3 [J].

Alexopoulou, L ;

Holt, AC ;

Medzhitov, R ;

Flavell, RA .

NATURE, 2001, 413 (6857) :732-738

[3]

OPTIMIZED SURVIVAL OF HIPPOCAMPAL-NEURONS IN B27-SUPPLEMENTED NEUROBASAL(TM), A NEW SERUM-FREE MEDIUM COMBINATION [J].

BREWER, GJ ;

TORRICELLI, JR ;

EVEGE, EK ;

PRICE, PJ .

JOURNAL OF NEUROSCIENCE RESEARCH, 1993, 35 (05) :567-576

[4]

TLR3 is an endogenous sensor of tissue necrosis during acute inflammatory events [J].

Cavassani, Karen A. ;

Ishii, Makoto ;

Wen, Haitao ;

Schaller, Matthew A. ;

Lincoln, Pamela M. ;

Lukacs, Nicholas W. ;

Hogaboam, Cory M. ;

Kunkel, Steven L. .

JOURNAL OF EXPERIMENTAL MEDICINE, 2008, 205 (11) :2609-2621

[5]

Chloroquine pretreatment inhibits toll-like receptor 3 signaling after stroke [J].

Cui, Guiyun ;

Ye, Xinchun ;

Zuo, Tao ;

Zhao, Hui ;

Zhao, Qiuchen ;

Chen, Wei ;

Hua, Fang .

NEUROSCIENCE LETTERS, 2013, 548 :101-104

[6]

LIPOFECTION - A HIGHLY EFFICIENT, LIPID-MEDIATED DNA-TRANSFECTION PROCEDURE [J].

FELGNER, PL ;

GADEK, TR ;

HOLM, M ;

ROMAN, R ;

CHAN, HW ;

WENZ, M ;

NORTHROP, JP ;

RINGOLD, GM ;

DANIELSEN, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (21) :7413-7417

[7]

Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans [J].

Fire, A ;

Xu, SQ ;

Montgomery, MK ;

Kostas, SA ;

Driver, SE ;

Mello, CC .

NATURE, 1998, 391 (6669) :806-811

[8]

Chemokines in ischemia and reperfusion [J].

Frangogiannis, Nikolaos G. .

THROMBOSIS AND HAEMOSTASIS, 2007, 97 (05) :738-747

[9]

RNA interference reveals a requirement for myocyte enhancer factor 2A in activity-dependent neuronal survival [J].

Gaudillière, B ;

Shi, Y ;

Bonni, A .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (48) :46442-46446

[10]

Towards safe, non-viral therapeutic gene expression in humans [J].

Glover, DJ ;

Lipps, HJ ;

Jans, DA .

NATURE REVIEWS GENETICS, 2005, 6 (04) :299-U29

← 1 2 3 4 5 6 →