Streptomyces spores from mouldy houses induce nitric oxide, TNF alpha and IL-6 secretion from RAW264.7 macrophage cell line without causing subsequent cell death

被引:43
作者
Hirvonen, MR
Nevalainen, A
Makkonen, N
Monkkonen, J
Savolainen, K
机构
[1] UNIV KUOPIO,DEPT PHARMACEUT,FIN-70211 KUOPIO,FINLAND
[2] UNIV KUOPIO,DEPT PHARMACOL & TOXICOL,FIN-70211 KUOPIO,FINLAND
基金
芬兰科学院;
关键词
cytokines; NO; cytotoxicity; indoor air; microorganisms;
D O I
10.1016/S1382-6689(96)00140-8
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The current view is that only bacterial lipopolysaccharide (LPS) and gamma interferon (IFN gamma) are able to alone activate macrophages to secrete nitric oxide (NO), probably a causative agent of cell death. Moreover, some cytokines and gram positive pathogens together with IFN gamma induce NO-production. Surprisingly, spores of Streptomyces sp., which are mesophilic gram-positive bacteria found in mouldy houses, stimulated RAW264.7 macrophages to produce pro-inflammatory cytokines, tumor necrosis factor alpha (TNF alpha) and interleukin-6 (IL-6), and induced the expression of inducible NO-synthase (iNOS) with a subsequent NO-production. However, the Streptomyces spores did not kill NO-producing macrophages, as did both LPS and gram negative bacteria Pseudomonas fluorescens, strong inducers of cytokine- and NO-production. These results imply that Streptomyces sp., induced cytokine and NO-secretion, may play a role in the responses evoked by exposure to these microbes. Moreover, factors other than, or in addition to NO, are necessary for cytotoxicity in murine macrophages. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:57 / 63
页数:7
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