Cloning of cDNA for goldfish activin beta(B) subunit, and the expression of its mRNA in gonadal and non-gonadal tissues

被引：31

作者：

Ge, W

Miura, T

Kobayashi, H

Peter, RE

Nagahama, Y

机构：

[1] NATL INST BASIC BIOL,DEPT DEV BIOL,REPROD BIOL LAB,OKAZAKI,AICHI 444,JAPAN

[2] UNIV ALBERTA,DEPT SCI BIOL,EDMONTON,AB T6G 2E9,CANADA

来源：

JOURNAL OF MOLECULAR ENDOCRINOLOGY | 1997年 / 19卷 / 01期

关键词：

D O I：

10.1677/jme.0.0190037

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We have cloned a full length cDNA coding for activin beta(B) subunit from the goldfish ovary. Sequence analysis of the goldfish activin beta(B) shows that this peptide is extremely conserved across vertebrates. The mature region of goldfish activin beta(B) has 93 and 98% amino acid identity with that of human and zebrafish beta(B) subunit respectively. The identity of the cloned goldfish activin beta(B) was further confirmed by expressing the protein in the Chinese hamster ovary (CHO) cells followed by detection of the specific activity of activin in the culture medium using F5-5 cell assay. mRNA of goldfish activin beta(B) is expressed in a variety of goldfish tissues including ovary, testis, brain, pituitary, kidney and liver, suggesting a wide range of physiological roles for activin in the goldfish.

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页码：37 / 45

页数：9

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