Determination of cyclosporin A in 20% ethanol by a magnetic beads-based immunofluorescence assay

被引：22

作者：

Kiselev, MV

Gladilin, AK ^{[1
]}

Melik-Nubarov, NS

Sveshnikov, PG

Miethe, P

Levashov, AV

机构：

[1] Moscow MV Lomonosov State Univ, Dept Chem, Moscow 119899, Russia

[2] ABION, Gesselschaft Biowissensch & Tech mbH, D-52428 Juelich, Germany

来源：

ANALYTICAL BIOCHEMISTRY | 1999年 / 269卷 / 02期

关键词：

D O I：

10.1006/abio.1999.4015

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid magnetic beads-based immunoassay for the immunodepressant drug cyclosporin A (CsA) has been developed. The method allows CsA determination in medium with a higher content of ethanol compared to conventional immunochemical techniques due to increased antibody stability. Monitoring of the drug in ethanol extracts from patient's whole blood without many-fold dilution with aqueous buffer is possible. The assay has adequate specificity and sensitivity for CsA to be suitable for the routine monitoring of therapy, (C) 1999 Academic Press.

引用

页码：393 / 398

页数：6

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