HBV promotes the proliferation of hepatic stellate cells via the PDGF-B/PDGFR-β signaling pathway in vitro

被引：71

作者：

Bai, Qixuan ^{[1
]}

An, Jing ^{[2
]}

Wu, Xiaoning ^{[1
]}

You, Hong ^{[1
]}

Ma, Hong ^{[1
]}

Liu, Tianhui ^{[1
]}

Gao, Na ^{[2
]}

Jia, Jidong ^{[1
]}

机构：

[1] Capital Med Univ, Liver Res Ctr, Beijing Friendship Hosp, Beijing 100050, Peoples R China

[2] Capital Med Univ, Sch Basic Med Sci, Dept Microbiol, Beijing 100050, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | 2012年 / 30卷 / 06期

基金：

国家高技术研究发展计划(863计划); 中国国家自然科学基金;

关键词：

hepatitis B virus; hepatic stellate cells; liver fibrosis; platelet-derived growth factor; FAT-STORING CELLS; GROWTH FACTOR-BB; LIVER FIBROSIS; KINASE INHIBITOR; RECEPTOR-BETA; VIRUS; EXPRESSION; ACTIVATION; FIBROGENESIS; ALPHA;

D O I：

10.3892/ijmm.2012.1148

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

100103 [病原生物学]; 100218 [急诊医学];

摘要：

The activation of hepatic stellate cells (HSCs) is closely associated with liver fibrosis in chronic hepatitis B virus (HBV) infection. However, the molecular mechanisms leading to HSC activation remain unclear. It has been reported that the platelet-derived growth factor-B (PDGF-13)/PDGF receptor-beta (PDGFR-beta) signaling pathway is involved in this process. Thus, we investigated whether HBV and its protein contribute to HSC proliferation by the PDGF-B/PDGFR-beta signaling pathway. HBV particles were purified from the supernatant of HepG2.2.15 cells by ultracentrifugation and the cell lines carrying HBV preS, e, c or x genes were obtained. After incubation with HBV particles or co-cultured with the cell lines expressed in the viral protein, the proliferation of LX-2 cells, an HSC cell line, were detected by flow cytometry and real-time PCR and the expression of molecules related to the PDGF-B/PDGFR-beta signaling pathway were further measured. Our results indicated that HBV particles, c and x proteins promoted LX-2 proliferation and increased the mRNA levels of PDGF-B, PDGFR-beta, collagen-1 and alpha-smooth muscle actin (alpha-SMA), as well as the phosphorylation of PDGFR-beta; however, the expression protein levels of PDGF-B and PDGFR-beta remained unchanged. In conclusion, HBV particles and HBV c and x proteins promote HSC proliferation and fibrogenesis in vitro and the PDGF-beta/PDGFR-beta signaling pathway is important in this process.

引用

页码：1443 / 1450

页数：8

共 31 条

[1]

Pro-fibrogenic potential of PDGF-D in liver fibrosis [J].