Optimization of hydroxyl radical formation using TiO2 as photocatalyst by response surface methodology

The determination of the optimum parameters for hydroxyl radicals ((OH)-O-center dot) formation by a TiO2 solution has been investigated by measuring the emitted fluorescence after the reaction with terephthalic acid has occurred. After UV irradiation, the terephthalic acid was transformed into 2-hydroxyterephthalic acid whose fluorescence is directly proportional to the generated (OH)-O-center dot. Optimization of hydroxyl radicals' formation using TiO2 as catalyst was carried out by studying the effects of irradiation time, TiO2 concentration and terephthalic acid concentration on the production of the fluorescent HTA with an experimental design. The aim Of our research was to apply response surface methodology as a chemometric method for the optimization of the reaction conditions. The combination of irradiation time, TiO2 concentration and terephthalic acid concentration was varied at designed points of a central composite rotatable design. The three factors were found to have a significant effect upon the reaction. The Optimum conditions for the reaction achievement were estimated to be 10 min for the irradiation time 25 mu g mL(-1) TiO2 concentration and 0.1 mmol L-1 terephthalic acid concentration. Afterwards, using these parameters the method was applied for the determination of the ability of several plant extract samples to scavenge the formed (OH)-O-center dot. (C) 2008 Elsevier B.V. All rights reserved.