Puerarin inhibits caspase-3 expression in osteoblasts of diabetic rats

被引：39

作者：

Liang, Junbo ^{[1
]}

Chen, Haixiao ^{[1
]}

Pan, Weibo ^{[1
]}

Xu, Chunli ^{[1
]}

机构：

[1] Taizhou Hosp Zhejiang Prov, Dept Orthoped, Taizhou 317000, Zhejiang, Peoples R China

来源：

MOLECULAR MEDICINE REPORTS | 2012年 / 5卷 / 06期

关键词：

puerarin; diabetic osteoporosis; caspase-3; bone mineral density; VERTEBRAL FRACTURES; BONE-FORMATION; MELLITUS; OSTEOPOROSIS; ACTIVATION; PI3K/AKT; DISEASE; KINASE; CELLS; FATE;

D O I：

10.3892/mmr.2012.854

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

The aim of this study was to investigate the protective effect of puerarin on attenuating caspase-3 expression in osteoblasts of streptozotocin-induced diabetic rats and the possible mechanisms involved. Diabetic rats were treated with a puerarin injection of 100 mg/kg/day. After 6 weeks, the bone mineral density, pathological changes and expression of caspase-3 were observed. The bone mineral density in the diabetic rats was significantly lower compared to the control group, while osteoblast numbers in the cortical bone were significantly reduced, and new bone formation in the diabetic rats was rare. Caspase-3 expression in the osteoblasts of diabetic rats increased compared to the rats in the control group. These pathological changes were improved and caspase-3 expression decreased in the puerarin-treated rats compared to the diabetic rats (P<0.01). High glucose levels affect bone metabolism and increase caspase-3 expression in osteoblasts, and thus lead to diabetic osteoporosis. Puerarin may play a protective role in diabetic osteoporosis via the reduction of caspase-3 expression.

引用

页码：1419 / 1422

页数：4

共 32 条

[1]

The effect of diabetes mellitus on rat mandibular bone formation and microarchitecture [J].

Abbassy, Mona A. ;

Watari, Ippei ;

Soma, Kunimichi .

EUROPEAN JOURNAL OF ORAL SCIENCES, 2010, 118 (04) :364-369

[2]

Reversal of redox-dependent inhibition of diacylglycerol kinase by antioxidants in mesangial cells exposed to high glucose [J].

Atsumi, Hisanari ;

Kitada, Munehiro ;

Kanasaki, Keizo ;

Koya, Daisuke .

MOLECULAR MEDICINE REPORTS, 2011, 4 (05) :923-927

[3]

The fate of circulating osteoblasts [J].

Canalis, E .

NEW ENGLAND JOURNAL OF MEDICINE, 2005, 352 (19) :2014-2016

[4]

Apoptotic signaling in methylglyoxal-treated human osteoblasts involves oxidative stress, c-jun N-terminal kinase, caspase-3, and p21-activated kinase 2 [J].

Chan, Wen-Hsiung ;

Wu, Hsin-Jung ;

Shiao, Nion-Heng .

JOURNAL OF CELLULAR BIOCHEMISTRY, 2007, 100 (04) :1056-1069

[5]

Neuroprotective mechanisms of puerarin in middle cerebral artery occlusion-induced brain infarction in rats [J].

Chang, Yi ;

Hsieh, Cheng-Ying ;

Peng, Zi-Aa ;

Yen, Ting-Lin ;

Hsiao, George ;

Chou, Duen-Suey ;

Chen, Chien-Ming ;

Sheu, Joen-Rong .

JOURNAL OF BIOMEDICAL SCIENCE, 2009, 16

[6]

Mediation of β-endorphin by the isoflavone puerarin to lower plasma glucose in streptozotocin-induced diabetic rats [J].

Chen, WC ;

Hayakawa, S ;

Yamamoto, T ;

Su, HC ;

Liu, IM ;

Cheng, JT .

PLANTA MEDICA, 2004, 70 (02) :113-116

[7]

Dexamethasone induces caspase activation in murine osteoblastic MC3T3-E1 cells [J].

Chua, CC ;

Chua, BHL ;

Chen, ZY ;

Landy, C ;

Hamdy, RC .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2003, 1642 (1-2) :79-85

[8]

Antioxidative and hypocholesterolemic activities of water-soluble puerarin glycosides in HepG2 cells and in C57BL/6J mice [J].

Chung, Mi Ja ;

Sung, Nak-Ju ;

Park, Cheon-Seok ;

Kweon, Dong-Keon ;

Mantovani, Alberto ;

Moon, Tae-Wha ;

Lee, Sung-Joon ;

Park, Kwan Hwa .

EUROPEAN JOURNAL OF PHARMACOLOGY, 2008, 578 (2-3) :159-170

[9]

Del Canizo Gomez Francisco Javier, 2011, Endocrinol Nutr, V58, P163, DOI 10.1016/j.endonu.2011.01.006

[10]

Influence of diabetic metabolic state on fracture heating in spontaneously diabetic rats [J].

Follak, N ;

Klöting, I ;

Merk, H .

DIABETES-METABOLISM RESEARCH AND REVIEWS, 2005, 21 (03) :288-296

← 1 2 3 4 →