Probing single-cell micromechanics in vivo:: The microrheology of C-elegans developing embryos

被引:133
作者
Daniels, Brian R.
Masi, Byron C.
Wirtz, Denis
机构
[1] Johns Hopkins Univ, Dept Chem & Biomol Engn, Baltimore, MD 21218 USA
[2] Johns Hopkins Univ, Dept Mat Sci & Engn, Baltimore, MD 21218 USA
[3] Johns Hopkins Univ, Howard Hughes Med Inst, Grad Program, Baltimore, MD 21218 USA
关键词
D O I
10.1529/biophysj.105.080606
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Cells are not directly accessible in vivo and therefore their mechanical properties cannot be measured by methods that require a direct contact between probe and cell. Here, we introduce a novel in vivo assay based on particle tracking microrheology whereby the extent and time-lag dependence of the mean squared displacements of thermally excited nanoparticles embedded within the cytoplasm of developing embryos reflect local viscoelastic properties. As a proof of principle, we probe local viscoelastic properties of the cytoplasm of developing Caenorhabditis elegans embryos. Our results indicate that unlike differentiated cells, the cytoplasm of these embryos does not exhibit measurable elasticity, but is highly viscous. Furthermore, the viscosity of the cytoplasm does not vary along the anterior-posterior axis of the embryo during the first cell division. These results support the hypothesis that the asymmetric positioning of the mitotic spindle stems from an asymmetric distribution of elementary force generators as opposed to asymmetric viscosity of the cytoplasm.
引用
收藏
页码:4712 / 4719
页数:8
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