A new method for the determination of electrical properties of supported bilayer lipid membranes by cyclic voltammetry

被引：39

作者：

Gu, LQ ^{[1
]}

Wang, LG ^{[1
]}

Xun, J ^{[1
]}

OttovaLeitmannova, A ^{[1
]}

Tien, HT ^{[1
]}

机构：

[1] MICHIGAN STATE UNIV, DEPT PHYSIOL, MEMBRANE BIOPHYS LAB, E LANSING, MI 48824 USA

来源：

BIOELECTROCHEMISTRY AND BIOENERGETICS | 1996年 / 39卷 / 02期

关键词：

bilayer membranes; cyclic voltammetry; electrical resistance; capacitance;

D O I：

10.1016/0302-4598(95)05035-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel method has been developed for accurate measurements of the membrane capacitance and resistance on the solid-supported bilayer lipid membrane (s-BLM) by the cyclic voltammetry (CV) technique. An improved equivalent circuit for the s-BLM is proposed, which is based on the solution of the coupled equations. This approach greatly facilitates the determination of the s-BLM electrical properties by CV. The curve-fitting procedure for testing the reliability of this method has been elaborated using a newly designed BLM simulator as well as a 50-point goodness-of-fit test. The mechanics and characteristics of formation of different s-BLMs and the acceleration effect of the CV potential on the s-BLM formation have been studied using the newly proposed s-BLM model.

引用

页码：275 / 283

页数：9

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