Adhesion of Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa onto nanohydroxyapatite as a bone regeneration material

被引:22
作者
Grenho, L. [1 ,2 ]
Manso, M. C. [3 ,5 ]
Monteiro, F. J. [1 ,2 ]
Ferraz, M. P. [1 ,4 ]
机构
[1] Univ Porto, Inst Engn Biomed, INEB, P-4150180 Oporto, Portugal
[2] Univ Porto, Dept Engn Met & Mat, Fac Engn FEUP, P-4200465 Oporto, Portugal
[3] Univ Fernando Pessoa, Fac Ciencias Saude, P-4249004 Oporto, Portugal
[4] Univ Fernando Pessoa, Ctr Estudos Biomed, CEBIMED, P-4249004 Oporto, Portugal
[5] Univ Porto, REQUIMTE, P-4100 Oporto, Portugal
关键词
bone infection; biomaterials; nanohydroxyapatite; bacterial adhesion; BIOFILMS; HYDROXYAPATITE; MICROSPHERES; INFECTIONS; FILMS;
D O I
10.1002/jbm.a.34139
中图分类号
R318 [生物医学工程];
学科分类号
100103 [病原生物学];
摘要
In orthopedics due to the enormous number of surgical procedures involving invasive implant biomaterials, infections have a huge impact in terms of morbidity, mortality, and medical costs. In this study the initial adhesion of several strains namely Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa, to nanohydroxyapatite, previously heat-treated at 725 degrees C and 1000 degrees C was assessed. Adherent cells were evaluated by scanning electron microscopy and quantified by confocal laser scanning microscopy and as colony forming units after being released by sonication. The wettability and roughness of samples surfaces were assessed by contact angle measurements and atomic force microscopy, respectively. Nanohydroxyapatite heat-treated at 1000 degrees C appeared to be more resistant to bacterial adhesion, over time, in five of the six tested strains while the clinical strains isolated from orthopedic infections presented superior ability to adhere, as well as better capacity to produce slime. The increase in materials sintering temperature resulted in increased hydrophobicity and roughness; however, other surface features such as the decrease in surface area and on porosity as well as the decrease on zeta potential may be the aspects that contributed to a lower bacterial adhesion on the materials sintered at 1000 degrees C. (C) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.
引用
收藏
页码:1823 / 1830
页数:8
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