Application of fungal and bacterial production methodologies to decomposing leaves in streams

被引:109
作者
Suberkropp, K
Weyers, H
机构
关键词
D O I
10.1128/AEM.62.5.1610-1615.1996
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
As leaves enter woodland streams, they are colonized by both fungi and bacteria, To determine the contribution of each of these microbial groups to the decomposition process, comparisons of fungal and bacterial production are needed. Recently, a new method for estimating fungal production based on rates of [C-14]acetate incorporation into ergosterol was described, Bacterial production in environmental samples has been determined from rates of [H-3]leucine incorporation into protein, In this study, we evaluated conditions necessary to use these methods for estimating fungal and bacterial production associated with leaves decomposing in a stream, During incubation of leaf disks with radiolabeled substrates, aeration increased rates of fungal incorporation but decreased bacterial production, Incorporation of both radiolabeled substrates by microorganisms associated with leaf litter was linear over the time periods examined (2 h for bacteria and 4 h for fungi). Incorporation of radiolabeled substrates present at different concentrations indicated that 400 nM leucine and 5 mM acetate maximized uptake for bacteria and fungi, respectively. Growth rates and rates of acetate incorporation into ergosterol followed similar patterns when fungi were grown on leaf disks in tile laboratory, Three species of stream fungi exhibited similar ratios of rates of biomass increase to rates of acetate incorporation into ergosterol, with a mean of 19.3 mu g of biomass per nmol of acetate incorporated, Both bacterial and fungal production increased exponentially with increasing temperature, In the stream that we examined, fungal carbon production was 11 to 26 times greater than bacterial carbon production on leaves colonized for 21 days.
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页码:1610 / 1615
页数:6
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