The influence of subinhibitory concentrations of ampicillin and vancomycin on physico-chemical surface characteristics of Enterococcus faecalis 1131

The effects of growth in the presence of sub-inhibitory concentrations of ampicillin and vancomycin on physicochemical cell surface properties of Enterococcus jaecalis 1131 have been determined. Growth in the presence of antibiotics yielded increased exposure of nitrogen and oxygen at the cell surface, proportional to the amount of antibiotic added to the growth medium, probably as a result of progressive removal of lipoteichoic acid (LTA) by ampicillin and vancomycin. Bacterial isoelectric points (IEP's), derived from particulate microelectrophoresis at different suspension pH, increased concurrently. Water contact angles on bacterial lawns only varied slightly in the presence of antibiotic during growth, whereas formamide contact angles after growth in the presence of vancomycin were significantly higher than after growth in the absence of this antibiotic, yielding a strongly electron-donating character of the cell surface. Surface thermodynamical analyses indicated favourable conditions for adhesion to hexadecane and chloroform, but more negative values of interaction free energies did not necessarily coincide with increased adhesion. Bacterial adhesion to hexadecane and chloroform increased when the pH of the bacterial suspension approached the isoelectric point of the organisms, because of the minimal electrostatic interactions. (C) 2002 Elsevier Science B.V. All rights reserved.