Development of an ultrasensitive immunoassay for rapid measurement of okadaic acid and its isomers

被引：33

作者：

Kreuzer, MP ^{[1
]}

O'Sullivan, CK ^{[1
]}

Guilbault, GG ^{[1
]}

机构：

[1] NUIC, Lab Sensor Dev, Dept Analyt Chem, Cork, Ireland

来源：

ANALYTICAL CHEMISTRY | 1999年 / 71卷 / 19期

关键词：

D O I：

10.1021/ac9901642

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This report highlights the characteristics of an okadaic acid immunoassay with limits of detection in the subfemtomole range. Two different immunoassay formats were investigated and their characteristics compared in relation to linear ranges, limits of detection, and cross-reactivity with other seafood toxins present in water and/or mussel samples. The developed ELISA system can be manipulated to quantitatively measure total diarrhetic shellfish poisoning (DSP) content or for okadaic acid and dinophysistoxin-1 individual concentrations by variation of the format of the immunoassay. Real mussel samples were validated in percentage recovery test. Calibration curves were established, and aliquots of real samples were tested. Very good recoveries were attained, highlighting the validity of the ELISA system to accurately determine the DSP concentration in mussel samples.

引用

页码：4198 / 4202

页数：5

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