A New Loop-Mediated Isothermal Amplification Method for Rapid, Simple, and Sensitive Detection of Leptospira spp. in Urine

被引：64

作者：

Koizumi, Nobuo ^{[1
]}

Nakajima, Chie ^{[2
]}

Harunari, Tsunehito ^{[3
]}

Tanikawa, Tsutomu ^{[3
]}

Tokiwa, Toshihiro ^{[4
]}

Uchimura, Eriko ^{[5
]}

Furuya, Tokujiro ^{[6
]}

Mingala, Claro Niegos ^{[7
]}

Villanueva, Marvin Ardeza ^{[7
]}

Ohnishi, Makoto ^{[1
]}

Suzuki, Yasuhiko ^{[2
]}

机构：

[1] Natl Inst Infect Dis, Dept Bacteriol, Tokyo, Japan

[2] Hokkaido Univ, Res Ctr Zoonosis Control, Div Global Epidemiol, Sapporo, Hokkaido 060, Japan

[3] Ikari Corp, Chiba, Japan

[4] Tokyo Med & Dent Univ, Grad Sch, Sect Environm Parasitol, Tokyo, Japan

[5] Kagoshima Cent Livestock Hyg Serv Ctr, Kagoshima, Japan

[6] Pfizer Japan Inc, Tokyo, Japan

[7] Philippine Carabao Ctr Natl Headquarters & Genepo, Anim Hlth Unit, Nueva Ecija, Philippines

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2012年 / 50卷 / 06期

关键词：

EARLY-DIAGNOSIS; LAMP;

D O I：

10.1128/JCM.00481-12

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We developed a new loop-mediated isothermal amplification (LAMP) method to detect rrs, a 16S rRNA gene of pathogenic Leptospira spp. in urine. The method enables detection of two leptospiral cells per reaction mixture following boiling of urine specimens. The sensitivity of this method is higher than that of culture or of flaB nested PCR.

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收藏

页码：2072 / 2074

页数：3

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