Key interactions in HIV-1 maturation identified by hydrogen-deuterium exchange

被引：150

作者：

Lanman, J

Lam, TT

Emmett, MR

Marshall, AG

Sakalian, M

Prevelige, PE ^{[1
]}

机构：

[1] Univ Alabama, Dept Microbiol, Birmingham, AL 35294 USA

[2] Florida State Univ, Dept Chem & Biochem, Tallahassee, FL 32306 USA

[3] Florida State Univ, Natl High Magnet Field Lab, Tallahassee, FL 32310 USA

[4] Univ Oklahoma, Hlth Sci Ctr, Dept Microbiol & Immunol, Oklahoma City, OK 73190 USA

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2004年 / 11卷 / 07期

关键词：

D O I：

10.1038/nsmb790

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To characterize the intersubunit interactions underlying assembly and maturation in HIV-1, we determined the amide hydrogen exchange protection pattern of capsid protein in the immature virion and the mature virion using mass spectrometry. Alterations in protection upon maturation provide evidence for the maturation-induced formation of an interaction between the N- and C-terminal domains in half of the capsid molecules, indicating that only half of the capsid protein is assembled into the conical core.

引用

收藏

页码：676 / 677

页数：2

相关论文

共 17 条

[1] The stoichiometry of Gag protein in HIV-1 [J].

Briggs, JAG ;

Simon, MN ;

Gross, I ;

Kräusslich, HG ;

Fuller, SD ;

Vogt, VM ;

Johnson, MC .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2004, 11 (07) :672-675

[2] Structural organization of authentic, mature HIV-1 virions and cores [J].

Briggs, JAG ;

Wilk, T ;

Welker, R ;

Kräusslich, HG ;

Fuller, SD .

EMBO JOURNAL, 2003, 22 (07) :1707-1715

[3] FUNCTIONAL DOMAINS OF THE CAPSID PROTEIN OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 [J].

DORFMAN, T ;

BUKOVSKY, A ;

OHAGEN, A ;

HOGLUND, S ;

GOTTLINGER, HG .

JOURNAL OF VIROLOGY, 1994, 68 (12) :8180-8187

[4] Proline residues in the HIV-1NH2-terminal capsid domain:: Structure determinants for proper core assembly and subsequent steps of early replication [J].

Fitzon, T ;

Leschonsky, B ;

Bieler, K ;

Paulus, C ;

Schröder, J ;

Wolf, H ;

Wagner, R .

VIROLOGY, 2000, 268 (02) :294-307

[5] HIV-1 Gag proteins: Diverse functions in the virus life cycle [J].

Freed, EO .

VIROLOGY, 1998, 251 (01) :1-15

[6] Cryo-electron microscopy reveals ordered domains in the immature HIV-1 particle [J].

Fuller, SD ;

Wilk, T ;

Gowen, BE ;

Krausslich, HG ;

Vogt, VM .

CURRENT BIOLOGY, 1997, 7 (10) :729-738

[7] Assembly properties of the human immunodeficiency virus type 1 CA protein [J].

Ganser-Pornillos, BK ;

von Schwedler, UK ;

Stray, KM ;

Aiken, C ;

Sundquist, WI .

JOURNAL OF VIROLOGY, 2004, 78 (05) :2545-2552

[8] MACROMOLECULAR INTERACTIONS IN THE ASSEMBLY OF HIV AND OTHER RETROVIRUSES [J].

HUNTER, E .

SEMINARS IN VIROLOGY, 1994, 5 (01) :71-83

[9] Mapping of protein:protein contact surfaces by hydrogen/deuterium exchange, followed by on-line high-performance liquid chromatography-electrospray ionization Fourier-transform ion-cyclotron-resonance mass analysis [J].

Lam, TT ;

Lanman, JK ;

Emmett, MR ;

Hendrickson, CL ;

Marshall, AG ;

Prevelige, PE .

JOURNAL OF CHROMATOGRAPHY A, 2002, 982 (01) :85-95

[10] Identification of novel interactions in HIV-1 capsid protein assembly by high-resolution mass spectrometry [J].

Lanman, J ;

Lam, TT ;

Barnes, S ;

Sakalian, M ;

Emmett, MR ;

Marshall, AG ;

Prevelige, PE .

JOURNAL OF MOLECULAR BIOLOGY, 2003, 325 (04) :759-772