Determination of benflumetol in human plasma by reversed-phase high-performance liquid chromatography with ultraviolet detection

被引:39
作者
Zeng, MY [1 ]
Lu, ZL [1 ]
Yang, SC [1 ]
Zhang, M [1 ]
Liao, J [1 ]
Liu, SL [1 ]
Teng, XH [1 ]
机构
[1] NATL CTR BIOMED ANAL,BEIJING 100850,PEOPLES R CHINA
来源
JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1996年 / 681卷 / 02期
关键词
benflumetol;
D O I
10.1016/0378-4347(95)00542-0
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A reversed-phase high-performance liquid chromatographic method for the determination of benflumetol in human plasma is described. Benflumetol in plasma samples was extracted with a glacial acetic acid-ethyl acetate (1:100, v/v) mixture at pH 4.0. Chromatography was performed on a Spherisorb C-18 column using a methanol-water-glacial acetic acid-diethyl amine (93:6:1:0.03, v/v) mixture as the mobile phase and UV-VIS detection at 335 nm. The identity and purity of the benflumetol peak were carefully examined, and the internal standard method was applied for its quantitation. The absolute recovery of benflumetol in spiked plasma samples was 92.91% over the concentration range 5-4000 ng/ml. The recovery of internal standard ''8212'' at a concentration of 300 ng/ml in spiked plasma was 84.85%. The detection limit of benflumetol was 11.8 ng/ml. Plasma concentration-time profiles in healthy volunteer adults were measured after a single-dose oral administration of 500 mg of benflumetol. The assay procedures were within the quality control limits.
引用
收藏
页码:299 / 306
页数:8
相关论文
共 4 条
[1]  
Carmody W.R., 1961, Journal of Chemical Education, V38, P559, DOI DOI 10.1021/ED038P559
[2]  
DENG RX, 1989, CHIN J PHARM, V8, P372
[3]  
*HANGZH U DEP CHEM, 1982, HDB AN CHEM, V2, P27
[4]   ANALYSIS OF DRUGS AND OTHER TOXIC-SUBSTANCES IN BIOLOGICAL SAMPLES FOR PHARMACOKINETIC STUDIES [J].
PENG, GW ;
CHIOU, WL .
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1990, 531 :3-50