Listeria monocytogenes biofilm-associated protein (BapL) may contribute to surface attachment of L-monocytogenes but is absent from many field isolates

被引：55

作者：

Jordan, Suzanne J. ^{[1
]}

Perni, Stefano ^{[4
]}

Glenn, Sarah ^{[1
]}

Fernandes, Isabel ^{[2
]}

Barbosa, Manuela ^{[2
]}

Sol, Manuela ^{[2
]}

Tenreiro, Rogerio P. ^{[3
]}

Chambel, Lelia ^{[3
]}

Barata, Belarmino ^{[3
]}

Zilhao, Isabel ^{[3
]}

Aldsworth, Timothy G. ^{[4
]}

Adriao, Andreia ^{[5
]}

Faleiro, M. Leonor ^{[5
]}

Shama, Gilbert ^{[4
]}

Andrew, Peter W. ^{[1
]}

机构：

[1] Univ Leicester, Dept Infect Immun & Inflammat, Leicester LE1 9HN, Leics, England

[2] INETI DTIA, P-221649038 Lisbon, Portugal

[3] Inst Ciencia Aplicada & Tecnol, P-1749016 Lisbon, Portugal

[4] Univ Loughborough, Dept Chem Engn, Loughborough LE11 3TU, Leics, England

[5] Univ Algarve, FERN, IBB CBME, P-8000117 Faro, Portugal

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2008年 / 74卷 / 17期

关键词：

D O I：

10.1128/AEM.02419-07

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Listeria monocytogenes is a food-borne pathogen capable of adhering to a range of surfaces utilized within the food industry, including stainless steel. The factors required for the attachment of this ubiquitous organism to abiotic surfaces are still relatively unknown. In silico analysis of the L. monocytogenes EGD genome identified a putative cell wall-anchored protein (Lmo0435 [BapL]), which had similarity to proteins involved in biofilm formation by staphylococci. An insertion mutation was constructed in L. monocytogenes to determine the influence of this protein on attachment to abiotic surfaces. The results show that the protein may contribute to the surface adherence of strains that possess BapL, but it is not an essential requirement for all L. monocytogenes strains. Several BapL-negative field isolates demonstrated an ability to adhere to abiotic surfaces equivalent to that of BapL-positive strains. BapL is not required for the virulence of L. monocytogenes in mice.

引用

页码：5451 / 5456

页数：6

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