Comparative analysis of barley leaf proteome as affected by drought stress

被引:95
作者
Ashoub, Ahmed [1 ,2 ]
Beckhaus, Tobias [3 ]
Berberich, Thomas [1 ]
Karas, Michael [3 ]
Brueggemann, Wolfgang [1 ]
机构
[1] Biodivers & Climate Res Ctr BiK F, Frankfurt, Germany
[2] ARC, Agr Genet Engn Res Inst AGERI, Giza, Egypt
[3] Goethe Univ Frankfurt, Inst Pharmaceut Chem, D-60438 Frankfurt, Germany
关键词
Barley; Difference gel electrophoresis (DIGE); Drought stress; F-v/F-m; MALDI-TOF-MS; Performance index; Proteome analysis; WATER-STRESS; CYTOSOLIC/NUCLEAR HSC70; MOLECULAR CHAPERONES; RESPONSIVE PROTEINS; RUBISCO ACTIVITY; HORDEUM-VULGARE; OSMOTIC-STRESS; ABSCISIC-ACID; C-3; PLANTS; CELL-DEATH;
D O I
10.1007/s00425-012-1798-4
中图分类号
Q94 [植物学];
学科分类号
071001 [植物学];
摘要
The adaptive response of Egyptian barley land races to drought stress was analyzed using difference gel electrophoresis (DIGE). Physiological measurements and proteome alterations of accession number 15141, drought tolerant, and accession number 15163, drought sensitive, were compared. Differentially expressed proteins were subjected to MALDI-TOF-MS analysis. Alterations in proteins related to the energy balance and chaperons were the most characteristic features to explain the differences between the drought-tolerant and the drought-sensitive accessions. Further alterations in the levels of proteins involved in metabolism, transcription and protein synthesis are also indicated.
引用
收藏
页码:771 / 781
页数:11
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