Muscle-derived hematopoietic stem cells are hematopoietic in origin

被引:353
作者
McKinney-Freeman, SL
Jackson, KA
Camargo, FD
Ferrari, G
Mavilio, F
Goodell, MA
机构
[1] Baylor Coll Med, Dept Immunol, Ctr Cell & Gene Therapy, Houston, TX 77030 USA
[2] Baylor Coll Med, Dept Pediat, Houston, TX 77030 USA
[3] Baylor Coll Med, Program Mol & Cell Biol, Houston, TX 77030 USA
[4] Inst Sci H San Rafaele, H San Raffaele Telethon Inst Gene Therapy, I-20132 Milan, Italy
[5] Univ Modena, Sch Med, Dept Biomed Sci, I-41100 Modena, Italy
关键词
D O I
10.1073/pnas.032438799
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
it has recently been shown that mononuclear cells from murine skeletal muscle contain the potential to repopulate all major peripheral blood lineages in lethally irradiated mice, but the origin of this activity is unknown. We have fractionated muscle cells on the basis of hematopoietic markers to show that the active population exclusively expresses the hematopoietic stem cell antigens Sca-1 and CD45. Muscle cells obtained from 6- to 8-week-old C57BL/6-CD45.1 mice and enriched for cells expressing Sca-1 and CD45 were able to generate hematopoietic but not myogenic colonies in vitro and repopulated multiple hematopoietic lineages of lethally irradiated C57BL/6-CD45.2 mice. These data show that muscle-derived hematopoietic stem cells are likely derived from the hematopoietic system and are a result not of transdifferentiation of myogenic stem cells but instead of the presence of substantial numbers of hematopoietic stem cells in the muscle. Although CD45-negative cells were highly myogenic in vitro and in vivo, CD45-positive muscle-derived cells displayed only very limited myogenic activity and only in vivo.
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收藏
页码:1341 / 1346
页数:6
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