Tissue-inherent fate of GPI revealed by GPI-anchored GFP transgenesis

被引：40

作者：

Kondoh, G

Gao, XH

Nakano, Y

Koike, H

Yamada, S

Okabe, M

Takeda, J

机构：

[1] Osaka Univ, Grad Sch Med, Dept Social & Environm Med, Suita, Osaka 5650871, Japan

[2] Osaka Univ, Grad Sch Med, Dept Dermatol, Suita, Osaka 5650871, Japan

[3] Osaka Univ, Grad Sch Med, Dept Clin Neurosci, Suita, Osaka 5650871, Japan

[4] Osaka Univ, Genome Informat Res Ctr, Suita, Osaka 5650871, Japan

来源：

FEBS LETTERS | 1999年 / 458卷 / 03期

关键词：

glycosylphosphatidylinositol-anchor; enhanced green fluorescent protein; cell surface; transgenic mouse; polarization;

D O I：

10.1016/S0014-5793(99)01172-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To clarify the fate of glycosylphosphatidylinositol (GPI) in mammals, we developed GPI-anchored enhanced green fluorescent protein (EGFP-GPI) and transgenic mice carrying this fusion construct, When it was introduced to culture cells, the EGFP-GPI protein was correctly sorted to plasma membranes and microsomes depending on GPI biosynthesis. Transgenic mice carrying EGFP-GPI were found to show a broad transgene expression. Histologically, a prominent polarized localization of EGFP-GPI protein was observed in various epithelia, the nervous system and liver and secreted from some exocrine glands, as well as non-polarized presence in non-epithelial tissues, demonstrating a tissue-inherent manner of GPI sorting. (C) 1999 Federation of European Biochemical Societies.

引用

页码：299 / 303

页数：5

共 20 条

[1]

CHALFIE M, 1998, GREEN FLUORESSCENT P

[2] RELEASE OF DECAY-ACCELERATING FACTOR (DAF) FROM THE CELL-MEMBRANE BY PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE-C (PIPLC) - SELECTIVE MODIFICATION OF A COMPLEMENT REGULATORY PROTEIN [J].