Molecular cloning and characterization of a novel putative carboxylesterase, present in human intestine and liver

被引:104
作者
Schwer, H
Langmann, T
Daig, R
Becker, A
Aslanidis, C
Schmitz, G
机构
[1] UNIV REGENSBURG,INST CLIN CHEM & LAB MED,D-93042 REGENSBURG,GERMANY
[2] UNIV REGENSBURG,DEPT INTERNAL MED 1,D-93042 REGENSBURG,GERMANY
关键词
D O I
10.1006/bbrc.1997.6413
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A full-length cDNA coding for a putative intestinal carboxylesterase (iCE) was isolated from a human small intestine cDNA library. The cDNA has an open reading frame of 559 amino acids with up to 65 % homology to other carboxylesterases of different mammalian species, The deduced amino-acid sequence contains many structural features, that are highly conserved among all carboxylesterase isoenzymes, like the serine esterase active site, an ER-retention signal and one Asn-Xxx-Thr site for N-linked carbohydrate addition. Northern blot analysis revealed that the corresponding mRNA is 3.4-3.6 kb in size and is preferentially expressed in human intestine with a weak signal also in liver. Analysis of cells from the gastrointestinal tract unveiled site-specific, transcriptional regulation of iCE, with higher expression in small intestine and lower expression in colon and rectum. The high expression in small intestine is attributable to a higher expression in jejunum compared to duodenum and ileum. (C) 1997 Academic Press.
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页码:117 / 120
页数:4
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