The 105-kDa protein component of Bacillus cereus non-haemolytic enterotoxin (Nhe) is a metalloprotease with gelatinolytic and collagenolytic activity

被引:32
作者
Lund, T [1 ]
Granum, PE [1 ]
机构
[1] Norwegian Sch Vet Sci, Dept Pharmacol Microbiol & Food Hyg, N-0033 Oslo, Norway
关键词
food poisoning; enterotoxin; collagenase; Bacillus cereus;
D O I
10.1111/j.1574-6968.1999.tb08699.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A sequence of 91 amino acids residues, probably starting from the N-terminal of the mature protein, was determined for the 105-kDa protein of the non-haemolytic enterotoxin of Bacillus. The last part of this sequence was similar to parts of the N-terminal portions of two collagenases of Clostridium histolyticum and Clostridium perfringens. Zymography, with intact collagen Fibril and gelatin as substrates, showed that the 105-kDa protein had collagenolytic and gelatinolytic activity. The 105-kDa protein also showed activity against a typical collagenase substrate, azocoll, and was inhibited by EDTA and 1,10-phenanthroline. We conclude that the 105-kDa protein is a collagenase. (C) 1999 Federation of European Microbiological Societies.
引用
收藏
页码:355 / 361
页数:7
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