共 35 条
In vivo dissection of the Helicobacter pylori fur regulatory circuit by genome-wide location analysis
被引:78
作者:

Danielli, Alberto
论文数: 0 引用数: 0
h-index: 0
机构: Univ Bologna, Dept Biol, I-40126 Bologna, Italy

Roncarati, Davide
论文数: 0 引用数: 0
h-index: 0
机构: Univ Bologna, Dept Biol, I-40126 Bologna, Italy

Delany, Isabel
论文数: 0 引用数: 0
h-index: 0
机构: Univ Bologna, Dept Biol, I-40126 Bologna, Italy

Chiarini, Valentina
论文数: 0 引用数: 0
h-index: 0
机构: Univ Bologna, Dept Biol, I-40126 Bologna, Italy

Rappuoli, Rino
论文数: 0 引用数: 0
h-index: 0
机构: Univ Bologna, Dept Biol, I-40126 Bologna, Italy

Scarlato, Vincenzo
论文数: 0 引用数: 0
h-index: 0
机构: Univ Bologna, Dept Biol, I-40126 Bologna, Italy
机构:
[1] Univ Bologna, Dept Biol, I-40126 Bologna, Italy
[2] Chiron Vaccines, Mol Immunol Unit, Siena, Italy
关键词:
D O I:
10.1128/JB.00120-06
中图分类号:
Q93 [微生物学];
学科分类号:
071005 ;
100705 ;
摘要:
Iron homeostasis is particularly important in pathogenic bacteria, which need to compete with the host for this essential cofactor. In Helicobacter pylori, a causative agent of several gastric pathologies, iron uptake and storage genes are regulated at the transcriptional level by the ferric uptake regulator Fur. The regulatory circuit of Fur has recently come under focus because of an intimate interlink with a broader regulatory network governing metal homeostasis, acidic response, and virulence. To dissect the Fur regulatory circuit and identify in vivo targets of regulation, we developed a genome-wide location analysis protocol which allowed the identification of 200 genomic loci bound by Fur as well as the investigation of the binding efficiency of the protein to these loci in response to iron. Comparative analysis with transcriptomes of wild-type and fur deletion mutant strains allowed the distinction between targets associated with Fur regulation and genes indirectly influenced by the fur mutation. The Fur regulon includes 59 genes, 25 of which appear to be positively regulated. A case study conducted by primer extension analysis of two oppositely regulated genes, hpn2 and flaB, suggests that negative regulation as well as positive regulation occurs at the transcriptional level. Furthermore, the results revealed the existence of 13 Fur targeted loci within polycistronic operons, which were associated with transcript deregulation in the fur mutant strain. This study provides a systematic insight of Fur regulation at the genome-wide level in H. pylori and points to regulatory functions extending beyond the classical Fur repression paradigm.
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页码:4654 / 4662
页数:9
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