Expression of lux genes in a clinical isolate of Streptococcus pneumoniae:: Using bioluminescence to monitor gemifloxacin activity

被引：46

作者：

Beard, SJ

Salisbury, V

Lewis, RJ

Sharpe, JA

MacGowan, AP

机构：

[1] Univ W England, Fac Sci Appl, Bristol BS16 1QY, Avon, England

[2] Southmead Gen Hosp, Bristol Ctr Antimicrobial Res & Evaluat, Bristol, Avon, England

[3] Univ Leicester, Sch Med, Leicester, Leics, England

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2002年 / 46卷 / 02期

关键词：

D O I：

10.1128/AAC.46.2.538-542.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A clinical isolate of Streptococcus pneumoniae was transformed with a plasmid containing the lux operon of Photorhabdus luminescens that had been modified to function in gram-positive bacteria. Cells containing this plasmid produced light stably and constitutively, without compromising the growth rate. Light output was correlated with measurements of optical density, and viable counts during exponential growth and provided a sensitive, real-time measure of the pharmacodynamics of the fluoroquinolone gemifloxacin.

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页码：538 / 542

页数：5

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