Molecular cloning and functional expression of alternative oxidase from Candida albicans

被引：59

作者：

Huh, WK

Kang, SO ^{[1
]}

机构：

[1] Seoul Natl Univ, Biophys Lab, Seoul 151742, South Korea

[2] Seoul Natl Univ, Dept Microbiol, Seoul 151742, South Korea

[3] Seoul Natl Univ, Coll Nat Sci, Seoul 151742, South Korea

[4] Seoul Natl Univ, Res Ctr Mol Microbiol, Seoul 151742, South Korea

来源：

JOURNAL OF BACTERIOLOGY | 1999年 / 181卷 / 13期

关键词：

D O I：

10.1128/JB.181.13.4098-4102.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The AOX1 gene, which encodes an alternative oxidase, was isolated from the genomic DNA library of Candida albicans. The gene encodes a polypeptide consisting of 379 amino acids with a calculated molecular mass of 43,975 Da. The aox1/aox1 mutant strain did not show cyanide-resistant respiration under normal conditions but could still induce cyanide-resistant respiration when treated with antimycin A. The measurement of respiratory activity and Western blot analysis suggested the presence of another AOX. When C. albicans AOX1 was expressed in alternative oxidase-deficient Saccharomyces cerevisiae. it could confer cyanide-resistant respiration on S. cerevisiae.

引用

页码：4098 / 4102

页数：5

共 33 条

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